Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13250 | 3' | -50 | NC_003401.1 | + | 43477 | 0.66 | 0.994011 |
Target: 5'- -cAugUGCacAGGGGCGUGCGUgaGCu -3' miRNA: 3'- auUugACGcuUUCCCGCAUGUAg-CGu -5' |
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13250 | 3' | -50 | NC_003401.1 | + | 79774 | 0.66 | 0.994011 |
Target: 5'- cAGAUaGCGgcAGGGCGUguuccccggGCAaCGCAa -3' miRNA: 3'- aUUUGaCGCuuUCCCGCA---------UGUaGCGU- -5' |
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13250 | 3' | -50 | NC_003401.1 | + | 27716 | 0.66 | 0.994011 |
Target: 5'- -cGGCUGCG--GGGGCGgugGUUGCGu -3' miRNA: 3'- auUUGACGCuuUCCCGCaugUAGCGU- -5' |
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13250 | 3' | -50 | NC_003401.1 | + | 85644 | 0.67 | 0.984365 |
Target: 5'- cGGACgguccCGAAAGGGCGUgaaGCAUucCGCGc -3' miRNA: 3'- aUUUGac---GCUUUCCCGCA---UGUA--GCGU- -5' |
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13250 | 3' | -50 | NC_003401.1 | + | 8848 | 0.67 | 0.982344 |
Target: 5'- --cACUGCGGAAaccuGGG-GUACuUCGCGg -3' miRNA: 3'- auuUGACGCUUU----CCCgCAUGuAGCGU- -5' |
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13250 | 3' | -50 | NC_003401.1 | + | 117939 | 0.67 | 0.982344 |
Target: 5'- ----gUGCGcauGAGGGCGgcgGgGUCGCGg -3' miRNA: 3'- auuugACGCu--UUCCCGCa--UgUAGCGU- -5' |
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13250 | 3' | -50 | NC_003401.1 | + | 89513 | 0.68 | 0.972264 |
Target: 5'- aUAAACgggUGUGAgGAGGGCGUcuuuuuGCAUgGCAa -3' miRNA: 3'- -AUUUG---ACGCU-UUCCCGCA------UGUAgCGU- -5' |
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13250 | 3' | -50 | NC_003401.1 | + | 22145 | 0.68 | 0.9692 |
Target: 5'- -cAGCUGCGucuacgucGAGGGUGUuucuaaGUCGCAu -3' miRNA: 3'- auUUGACGCu-------UUCCCGCAug----UAGCGU- -5' |
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13250 | 3' | -50 | NC_003401.1 | + | 52811 | 0.69 | 0.962363 |
Target: 5'- -uAACUGCGGGaauaucAGGGCGcgACAUgGUAc -3' miRNA: 3'- auUUGACGCUU------UCCCGCa-UGUAgCGU- -5' |
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13250 | 3' | -50 | NC_003401.1 | + | 6530 | 0.69 | 0.958577 |
Target: 5'- aAGACUGUGGAGGGGCuGUcuCGUuccCGCGg -3' miRNA: 3'- aUUUGACGCUUUCCCG-CAu-GUA---GCGU- -5' |
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13250 | 3' | -50 | NC_003401.1 | + | 113275 | 0.7 | 0.94085 |
Target: 5'- gUGAACcGCGGAAaGGUGUGCAcaUCGUAa -3' miRNA: 3'- -AUUUGaCGCUUUcCCGCAUGU--AGCGU- -5' |
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13250 | 3' | -50 | NC_003401.1 | + | 36893 | 0.71 | 0.885264 |
Target: 5'- cGGACgaagGCGAGAGaGGCGUAuCAUUGUu -3' miRNA: 3'- aUUUGa---CGCUUUC-CCGCAU-GUAGCGu -5' |
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13250 | 3' | -50 | NC_003401.1 | + | 121470 | 0.73 | 0.836691 |
Target: 5'- gGGAUUGCgGAGGGGGCGaggACGUCgGCGu -3' miRNA: 3'- aUUUGACG-CUUUCCCGCa--UGUAG-CGU- -5' |
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13250 | 3' | -50 | NC_003401.1 | + | 113077 | 1.08 | 0.009459 |
Target: 5'- gUAAACUGCGAAAGGGCGUACAUCGCAg -3' miRNA: 3'- -AUUUGACGCUUUCCCGCAUGUAGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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