Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13250 | 5' | -50.3 | NC_003401.1 | + | 51760 | 0.66 | 0.992501 |
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Target: 5'- --aGCGAUGgcUGCGCGCaa--CCGCGa -3' miRNA: 3'- aaaUGCUAC--ACGUGUGgaaaGGCGCc -5' |
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| 13250 | 5' | -50.3 | NC_003401.1 | + | 63902 | 0.66 | 0.991833 |
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Target: 5'- -gUGCGcgGUGUugGCCagUaaccaccgcaacgggCCGCGGa -3' miRNA: 3'- aaAUGCuaCACGugUGGaaA---------------GGCGCC- -5' |
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| 13250 | 5' | -50.3 | NC_003401.1 | + | 55204 | 0.67 | 0.983499 |
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Target: 5'- gUUUugGGUuUGUACAgCg--CCGCGGg -3' miRNA: 3'- -AAAugCUAcACGUGUgGaaaGGCGCC- -5' |
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| 13250 | 5' | -50.3 | NC_003401.1 | + | 18910 | 0.67 | 0.981424 |
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Target: 5'- gUUACGGUgGUGCGC-CCgc-CCGgGGu -3' miRNA: 3'- aAAUGCUA-CACGUGuGGaaaGGCgCC- -5' |
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| 13250 | 5' | -50.3 | NC_003401.1 | + | 74532 | 0.7 | 0.93947 |
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Target: 5'- aUUACGGUGgacGagaGCGCCUUUCaCGCGc -3' miRNA: 3'- aAAUGCUACa--Cg--UGUGGAAAG-GCGCc -5' |
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| 13250 | 5' | -50.3 | NC_003401.1 | + | 118154 | 0.74 | 0.749558 |
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Target: 5'- --gACGGcaccaUGUGCACAUCUUUcagugacCCGCGGg -3' miRNA: 3'- aaaUGCU-----ACACGUGUGGAAA-------GGCGCC- -5' |
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| 13250 | 5' | -50.3 | NC_003401.1 | + | 113111 | 1.08 | 0.009232 |
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Target: 5'- uUUUACGAUGUGCACACCUUUCCGCGGu -3' miRNA: 3'- -AAAUGCUACACGUGUGGAAAGGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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