Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13251 | 3' | -45.4 | NC_003401.1 | + | 76176 | 0.66 | 0.999948 |
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Target: 5'- gUCUGCGUgCAUuaGAACAaaAUACUGUUu -3' miRNA: 3'- aAGAUGCAaGUG--CUUGU--UGUGACAAc -5' |
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| 13251 | 3' | -45.4 | NC_003401.1 | + | 77506 | 0.66 | 0.999948 |
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Target: 5'- gUUC-ACGUUCGCGAACAAaACUcguccagauGUUGu -3' miRNA: 3'- -AAGaUGCAAGUGCUUGUUgUGA---------CAAC- -5' |
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| 13251 | 3' | -45.4 | NC_003401.1 | + | 69748 | 0.68 | 0.999303 |
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Target: 5'- ---cGCGUUCGCGAGCAGgGCUu--- -3' miRNA: 3'- aagaUGCAAGUGCUUGUUgUGAcaac -5' |
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| 13251 | 3' | -45.4 | NC_003401.1 | + | 41259 | 0.69 | 0.998927 |
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Target: 5'- aUCUGCGUUCACcuGGGCAACGgUaGUa- -3' miRNA: 3'- aAGAUGCAAGUG--CUUGUUGUgA-CAac -5' |
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| 13251 | 3' | -45.4 | NC_003401.1 | + | 11140 | 0.7 | 0.998049 |
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Target: 5'- cUCUACGUguUCGCGAcugaGGCGCUGg-- -3' miRNA: 3'- aAGAUGCA--AGUGCUug--UUGUGACaac -5' |
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| 13251 | 3' | -45.4 | NC_003401.1 | + | 61777 | 0.7 | 0.996002 |
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Target: 5'- gUCUGCGUUgGCGAAcCGACGCa---- -3' miRNA: 3'- aAGAUGCAAgUGCUU-GUUGUGacaac -5' |
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| 13251 | 3' | -45.4 | NC_003401.1 | + | 113218 | 1.08 | 0.030276 |
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Target: 5'- gUUCUACGUUCACGAACAACACUGUUGa -3' miRNA: 3'- -AAGAUGCAAGUGCUUGUUGUGACAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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