Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13252 | 5' | -54.6 | NC_003401.1 | + | 40761 | 0.66 | 0.950582 |
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Target: 5'- aUACGGGCugGUGUacaacuaUUaccagggcaaaaacgUGGcacacGUUCCGGa -3' miRNA: 3'- -GUGCCCGugCACAg------AA---------------ACC-----CAAGGCC- -5' |
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| 13252 | 5' | -54.6 | NC_003401.1 | + | 17503 | 0.66 | 0.9489 |
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Target: 5'- aCGCGGcGCGCGUGgcccccGGGUUCgugcUGGa -3' miRNA: 3'- -GUGCC-CGUGCACagaaa-CCCAAG----GCC- -5' |
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| 13252 | 5' | -54.6 | NC_003401.1 | + | 85230 | 0.66 | 0.935072 |
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Target: 5'- aCACGGGUccccccGCG-GcCUUuguuaaacucUGGGUUUCGGg -3' miRNA: 3'- -GUGCCCG------UGCaCaGAA----------ACCCAAGGCC- -5' |
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| 13252 | 5' | -54.6 | NC_003401.1 | + | 18946 | 0.67 | 0.924651 |
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Target: 5'- gGCGGGUACuaUGUCcagUGGGUugUCgCGGu -3' miRNA: 3'- gUGCCCGUGc-ACAGaa-ACCCA--AG-GCC- -5' |
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| 13252 | 5' | -54.6 | NC_003401.1 | + | 7768 | 0.67 | 0.900917 |
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Target: 5'- uGCcaGUACGUGUaCUUUGGGUgcucgucggCCGGg -3' miRNA: 3'- gUGccCGUGCACA-GAAACCCAa--------GGCC- -5' |
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| 13252 | 5' | -54.6 | NC_003401.1 | + | 121154 | 0.68 | 0.866001 |
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Target: 5'- gGCGGGCGCGgggGgcgCgg-GGGgcgcgCCGGu -3' miRNA: 3'- gUGCCCGUGCa--Ca--GaaaCCCaa---GGCC- -5' |
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| 13252 | 5' | -54.6 | NC_003401.1 | + | 121634 | 0.69 | 0.825786 |
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Target: 5'- gGCGGuGCAgGUGag--UGGGggCCGGg -3' miRNA: 3'- gUGCC-CGUgCACagaaACCCaaGGCC- -5' |
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| 13252 | 5' | -54.6 | NC_003401.1 | + | 113418 | 1.11 | 0.002795 |
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Target: 5'- cCACGGGCACGUGUCUUUGGGUUCCGGg -3' miRNA: 3'- -GUGCCCGUGCACAGAAACCCAAGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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