Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13256 | 3' | -41 | NC_003401.1 | + | 69349 | 0.66 | 1 |
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Target: 5'- cAGGUGGAAGGGUccgcGGGCgucgaUUGCGg- -3' miRNA: 3'- -UUUACCUUUUUAau--UCCGa----AACGCaa -5' |
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| 13256 | 3' | -41 | NC_003401.1 | + | 77008 | 0.67 | 1 |
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Target: 5'- --uUGGGAAGAUUAuucuuGGUUUUGCu-- -3' miRNA: 3'- uuuACCUUUUUAAUu----CCGAAACGcaa -5' |
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| 13256 | 3' | -41 | NC_003401.1 | + | 64863 | 0.67 | 1 |
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Target: 5'- cGGUGGAAGAGggAAGGCg--GgGUc -3' miRNA: 3'- uUUACCUUUUUaaUUCCGaaaCgCAa -5' |
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| 13256 | 3' | -41 | NC_003401.1 | + | 100948 | 0.66 | 1 |
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Target: 5'- --cUGGAGcggGAGUUAAGaGUUUUGUGUc -3' miRNA: 3'- uuuACCUU---UUUAAUUC-CGAAACGCAa -5' |
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| 13256 | 3' | -41 | NC_003401.1 | + | 106111 | 0.66 | 1 |
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Target: 5'- cGGGUGGAGGGAUgcGGGUa--GCGUUu -3' miRNA: 3'- -UUUACCUUUUUAauUCCGaaaCGCAA- -5' |
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| 13256 | 3' | -41 | NC_003401.1 | + | 121416 | 0.69 | 0.999999 |
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Target: 5'- gGGAUGGGGccuGUUGAGGCguugGCGa- -3' miRNA: 3'- -UUUACCUUuu-UAAUUCCGaaa-CGCaa -5' |
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| 13256 | 3' | -41 | NC_003401.1 | + | 132763 | 0.69 | 0.999997 |
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Target: 5'- -cGUGGggGAGggGAGGCcg-GCGg- -3' miRNA: 3'- uuUACCuuUUUaaUUCCGaaaCGCaa -5' |
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| 13256 | 3' | -41 | NC_003401.1 | + | 114596 | 0.78 | 0.984715 |
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Target: 5'- --cUGGAAAAAUUAAGuGCccUUUGCGUUa -3' miRNA: 3'- uuuACCUUUUUAAUUC-CG--AAACGCAA- -5' |
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| 13256 | 3' | -41 | NC_003401.1 | + | 114400 | 1.05 | 0.105245 |
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Target: 5'- aAAAUGGAAAAAUUAAGGCUUUGCGUUa -3' miRNA: 3'- -UUUACCUUUUUAAUUCCGAAACGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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