Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13258 | 5' | -44.1 | NC_003401.1 | + | 23857 | 0.66 | 0.999995 |
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Target: 5'- -uUGGGuuUUGUUUacaAGUUCCCUAUa -3' miRNA: 3'- guGCCUuuAACAGAaa-UCAAGGGAUG- -5' |
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| 13258 | 5' | -44.1 | NC_003401.1 | + | 117590 | 0.66 | 0.999995 |
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Target: 5'- -uUGGGuuUUGUUUacaAGUUCCCUAUa -3' miRNA: 3'- guGCCUuuAACAGAaa-UCAAGGGAUG- -5' |
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| 13258 | 5' | -44.1 | NC_003401.1 | + | 85790 | 0.67 | 0.999981 |
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Target: 5'- aACGGAAAUUGaUCggUAGggaaUUCCUUAUc -3' miRNA: 3'- gUGCCUUUAAC-AGaaAUC----AAGGGAUG- -5' |
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| 13258 | 5' | -44.1 | NC_003401.1 | + | 18436 | 0.68 | 0.999973 |
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Target: 5'- gGCGGAG--UGUCacgug-UCCCUACg -3' miRNA: 3'- gUGCCUUuaACAGaaaucaAGGGAUG- -5' |
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| 13258 | 5' | -44.1 | NC_003401.1 | + | 113418 | 0.7 | 0.99914 |
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Target: 5'- cCACGGGcAcgUGUCUUUGGgUUCCggggGCg -3' miRNA: 3'- -GUGCCU-UuaACAGAAAUCaAGGGa---UG- -5' |
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| 13258 | 5' | -44.1 | NC_003401.1 | + | 113597 | 0.71 | 0.998691 |
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Target: 5'- -cUGGAGAcugUGUCUUUGGgUCCCUuccACg -3' miRNA: 3'- guGCCUUUa--ACAGAAAUCaAGGGA---UG- -5' |
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| 13258 | 5' | -44.1 | NC_003401.1 | + | 31692 | 0.77 | 0.954103 |
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Target: 5'- cCACGGAAucUGUCagUUGGUguaagCCCUGCc -3' miRNA: 3'- -GUGCCUUuaACAGa-AAUCAa----GGGAUG- -5' |
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| 13258 | 5' | -44.1 | NC_003401.1 | + | 114724 | 1.13 | 0.021747 |
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Target: 5'- aCACGGAAAUUGUCUUUAGUUCCCUACa -3' miRNA: 3'- -GUGCCUUUAACAGAAAUCAAGGGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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