Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13264 | 5' | -51.9 | NC_003401.1 | + | 118198 | 0.66 | 0.9702 |
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Target: 5'- gGCGGCGGcaccgcgggcgcUG--GGGGUGAUAUGUUCu -3' miRNA: 3'- aCGUCGCC------------ACagUCCCGUUGUAUAAG- -5' |
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| 13264 | 5' | -51.9 | NC_003401.1 | + | 6394 | 0.66 | 0.967014 |
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Target: 5'- aUGCGGCuuuuacGGUGagAGGGaauuCGUGUUCa -3' miRNA: 3'- -ACGUCG------CCACagUCCCguu-GUAUAAG- -5' |
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| 13264 | 5' | -51.9 | NC_003401.1 | + | 42488 | 0.67 | 0.959936 |
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Target: 5'- aUGCGuCGGgaaGUCAGGGCGgacGCAg--UCa -3' miRNA: 3'- -ACGUcGCCa--CAGUCCCGU---UGUauaAG- -5' |
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| 13264 | 5' | -51.9 | NC_003401.1 | + | 129461 | 0.67 | 0.956031 |
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Target: 5'- aUGCAcGCGG--UCAGGGCGAUuUGUa- -3' miRNA: 3'- -ACGU-CGCCacAGUCCCGUUGuAUAag -5' |
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| 13264 | 5' | -51.9 | NC_003401.1 | + | 106203 | 0.67 | 0.956031 |
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Target: 5'- aGCGGCGGaGUCGacgaccGGGCGGC---UUCg -3' miRNA: 3'- aCGUCGCCaCAGU------CCCGUUGuauAAG- -5' |
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| 13264 | 5' | -51.9 | NC_003401.1 | + | 112208 | 0.67 | 0.951875 |
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Target: 5'- cGUGGCGGcgcGUUAGGGCAgaaaaaaacuGCGUGUc- -3' miRNA: 3'- aCGUCGCCa--CAGUCCCGU----------UGUAUAag -5' |
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| 13264 | 5' | -51.9 | NC_003401.1 | + | 8512 | 0.68 | 0.932664 |
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Target: 5'- cGCGGCGGUcuugguGUCGGGG-AACAg---- -3' miRNA: 3'- aCGUCGCCA------CAGUCCCgUUGUauaag -5' |
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| 13264 | 5' | -51.9 | NC_003401.1 | + | 51148 | 0.69 | 0.888888 |
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Target: 5'- aUGCAGC-GUGUCcuGGGGCGAUAc---- -3' miRNA: 3'- -ACGUCGcCACAG--UCCCGUUGUauaag -5' |
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| 13264 | 5' | -51.9 | NC_003401.1 | + | 12988 | 0.69 | 0.874069 |
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Target: 5'- gGCGGcCGGUGUCGgccaaguucguGGGCGACGc---- -3' miRNA: 3'- aCGUC-GCCACAGU-----------CCCGUUGUauaag -5' |
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| 13264 | 5' | -51.9 | NC_003401.1 | + | 118254 | 0.89 | 0.0934 |
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Target: 5'- uUGCAGCGGUGUCAGcGG-AACAUAUUCu -3' miRNA: 3'- -ACGUCGCCACAGUC-CCgUUGUAUAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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