Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13265 | 3' | -57.1 | NC_003401.1 | + | 965 | 0.71 | 0.569114 |
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Target: 5'- gGCCCGggucCCGUGUCCUCGucUGGCCc -3' miRNA: 3'- -UGGGUaacuGGCGUAGGAGCc-ACCGG- -5' |
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| 13265 | 3' | -57.1 | NC_003401.1 | + | 133680 | 0.71 | 0.569114 |
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Target: 5'- gGCCCGggucCCGUGUCCUCGucUGGCCc -3' miRNA: 3'- -UGGGUaacuGGCGUAGGAGCc-ACCGG- -5' |
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| 13265 | 3' | -57.1 | NC_003401.1 | + | 116628 | 0.71 | 0.549049 |
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Target: 5'- gGCgCGUUGGCCGUAUUCUUGGUGa-- -3' miRNA: 3'- -UGgGUAACUGGCGUAGGAGCCACcgg -5' |
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| 13265 | 3' | -57.1 | NC_003401.1 | + | 79837 | 0.71 | 0.546058 |
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Target: 5'- uACCUcuucaccggaggcgGUUGcgGuuGCAgacgCCUCGGUGGCCg -3' miRNA: 3'- -UGGG--------------UAAC--UggCGUa---GGAGCCACCGG- -5' |
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| 13265 | 3' | -57.1 | NC_003401.1 | + | 128030 | 0.73 | 0.480877 |
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Target: 5'- cGCgCAccGGCaCGCcuGUCCUCGGUGGCUu -3' miRNA: 3'- -UGgGUaaCUG-GCG--UAGGAGCCACCGG- -5' |
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| 13265 | 3' | -57.1 | NC_003401.1 | + | 23986 | 0.74 | 0.399829 |
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Target: 5'- cGCCCAUUGGCUGCcgCCagCGuccaaUGGCCg -3' miRNA: 3'- -UGGGUAACUGGCGuaGGa-GCc----ACCGG- -5' |
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| 13265 | 3' | -57.1 | NC_003401.1 | + | 94560 | 0.75 | 0.350877 |
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Target: 5'- uGCCCAUUGAUCGU-UCCUCc-UGGCCg -3' miRNA: 3'- -UGGGUAACUGGCGuAGGAGccACCGG- -5' |
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| 13265 | 3' | -57.1 | NC_003401.1 | + | 118411 | 1.12 | 0.001262 |
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Target: 5'- gACCCAUUGACCGCAUCCUCGGUGGCCg -3' miRNA: 3'- -UGGGUAACUGGCGUAGGAGCCACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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