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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13267 | 5' | -57.6 | NC_003401.1 | + | 108306 | 0.66 | 0.817822 |
Target: 5'- cGUCGUCgUGCC-CUGuuUGGCGucGCCGGa -3' miRNA: 3'- -CAGCAG-ACGGaGAUccACCGC--UGGCU- -5' |
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13267 | 5' | -57.6 | NC_003401.1 | + | 120001 | 0.72 | 0.503736 |
Target: 5'- --gGUCUGCCcccgUCccuGGUGGCGGCCGc -3' miRNA: 3'- cagCAGACGG----AGau-CCACCGCUGGCu -5' |
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13267 | 5' | -57.6 | NC_003401.1 | + | 119562 | 1.08 | 0.00195 |
Target: 5'- uGUCGUCUGCCUCUAGGUGGCGACCGAa -3' miRNA: 3'- -CAGCAGACGGAGAUCCACCGCUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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