Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13268 | 3' | -56.4 | NC_003401.1 | + | 16663 | 0.66 | 0.868449 |
Target: 5'- aGGCUGCGGcgcGG-CUGUUgugcaagcggCCGCCGCa -3' miRNA: 3'- -UUGGCGCCuu-UCaGACAA----------GGCGGCGc -5' |
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13268 | 3' | -56.4 | NC_003401.1 | + | 46321 | 0.66 | 0.860853 |
Target: 5'- cGCCGCGuaccaGAuagcGUCaUGUgCCGCCGCGu -3' miRNA: 3'- uUGGCGC-----CUuu--CAG-ACAaGGCGGCGC- -5' |
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13268 | 3' | -56.4 | NC_003401.1 | + | 81979 | 0.66 | 0.853047 |
Target: 5'- gGGCCGUGGAGucgauaucagGGcCUGgcucgUUGCCGCGg -3' miRNA: 3'- -UUGGCGCCUU----------UCaGACaa---GGCGGCGC- -5' |
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13268 | 3' | -56.4 | NC_003401.1 | + | 117605 | 0.67 | 0.832657 |
Target: 5'- cGGCCGCGGcgaccaauggCCGCCGCGg -3' miRNA: 3'- -UUGGCGCCuuucagacaaGGCGGCGC- -5' |
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13268 | 3' | -56.4 | NC_003401.1 | + | 6531 | 0.67 | 0.828436 |
Target: 5'- aGACUGUGGAGGGgCUGUcUCGuucCCGCGg -3' miRNA: 3'- -UUGGCGCCUUUCaGACAaGGC---GGCGC- -5' |
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13268 | 3' | -56.4 | NC_003401.1 | + | 103775 | 0.67 | 0.792203 |
Target: 5'- cGGCCGCGGGucGUUcuaaacuUGUUCCcaCCGUGg -3' miRNA: 3'- -UUGGCGCCUuuCAG-------ACAAGGc-GGCGC- -5' |
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13268 | 3' | -56.4 | NC_003401.1 | + | 118423 | 0.68 | 0.783897 |
Target: 5'- gAGCCGgGGAGAauaUGUUCCGCUGaCa -3' miRNA: 3'- -UUGGCgCCUUUcagACAAGGCGGC-Gc -5' |
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13268 | 3' | -56.4 | NC_003401.1 | + | 117748 | 0.68 | 0.764094 |
Target: 5'- cGCCGCGGcc-GUCUGUcuuuugcUUCGCUGCc -3' miRNA: 3'- uUGGCGCCuuuCAGACA-------AGGCGGCGc -5' |
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13268 | 3' | -56.4 | NC_003401.1 | + | 55187 | 0.68 | 0.74572 |
Target: 5'- cGCCGCGGGGgcAGUCccaUCUGCCGUu -3' miRNA: 3'- uUGGCGCCUU--UCAGacaAGGCGGCGc -5' |
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13268 | 3' | -56.4 | NC_003401.1 | + | 12659 | 0.69 | 0.725986 |
Target: 5'- aAACCGaGGggGGUCUGUUUCuccuguggcaGCCGUu -3' miRNA: 3'- -UUGGCgCCuuUCAGACAAGG----------CGGCGc -5' |
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13268 | 3' | -56.4 | NC_003401.1 | + | 75808 | 0.69 | 0.715991 |
Target: 5'- cAGCUGCGGAGGGUUaa--CCGuuGCGg -3' miRNA: 3'- -UUGGCGCCUUUCAGacaaGGCggCGC- -5' |
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13268 | 3' | -56.4 | NC_003401.1 | + | 82363 | 0.69 | 0.705923 |
Target: 5'- cAGCUGUGGcacGAGuGUCUGUggcgggcguugUCCGCCGCu -3' miRNA: 3'- -UUGGCGCC---UUU-CAGACA-----------AGGCGGCGc -5' |
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13268 | 3' | -56.4 | NC_003401.1 | + | 47842 | 0.7 | 0.665109 |
Target: 5'- gGGCCGCGGGGacGGUCcg-UCCggGCCGUGg -3' miRNA: 3'- -UUGGCGCCUU--UCAGacaAGG--CGGCGC- -5' |
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13268 | 3' | -56.4 | NC_003401.1 | + | 122402 | 1.06 | 0.003664 |
Target: 5'- gAACCGCGGAAAGUCUGUUCCGCCGCGc -3' miRNA: 3'- -UUGGCGCCUUUCAGACAAGGCGGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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