Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13269 | 5' | -58.1 | NC_003401.1 | + | 19332 | 0.68 | 0.661317 |
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Target: 5'- aCGCCCGug--GUGUAcGUGGGCCUGAc -3' miRNA: 3'- -GCGGGCucgaCACGU-CGCUUGGACUu -5' |
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| 13269 | 5' | -58.1 | NC_003401.1 | + | 72384 | 0.68 | 0.661317 |
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Target: 5'- cCGUCCGcGCUG-GCAGCGAuuccGCCUc-- -3' miRNA: 3'- -GCGGGCuCGACaCGUCGCU----UGGAcuu -5' |
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| 13269 | 5' | -58.1 | NC_003401.1 | + | 15981 | 0.69 | 0.599356 |
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Target: 5'- aCGUUCGuGCUGaGCGGCGGACCg--- -3' miRNA: 3'- -GCGGGCuCGACaCGUCGCUUGGacuu -5' |
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| 13269 | 5' | -58.1 | NC_003401.1 | + | 78528 | 0.74 | 0.310728 |
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Target: 5'- cCGCCUG-GCggagGCGGCGAACCUGGc -3' miRNA: 3'- -GCGGGCuCGaca-CGUCGCUUGGACUu -5' |
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| 13269 | 5' | -58.1 | NC_003401.1 | + | 122597 | 0.79 | 0.164059 |
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Target: 5'- aGCCUuauaGuGCUGUGCAGCGAuCCUGAAa -3' miRNA: 3'- gCGGG----CuCGACACGUCGCUuGGACUU- -5' |
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| 13269 | 5' | -58.1 | NC_003401.1 | + | 123229 | 1.06 | 0.001995 |
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Target: 5'- cCGCCCGAGCUGUGCAGCGAACCUGAAg -3' miRNA: 3'- -GCGGGCUCGACACGUCGCUUGGACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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