Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13274 | 5' | -53.2 | NC_003409.1 | + | 106599 | 0.67 | 0.93191 |
Target: 5'- aCGGGuaGUGgcucuaccGCCUCUGC-AGGGCgUGAGg -3' miRNA: 3'- -GCUC--UAC--------CGGAGAUGuUUCCGgACUC- -5' |
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13274 | 5' | -53.2 | NC_003409.1 | + | 43336 | 0.67 | 0.926527 |
Target: 5'- uGAGAggGGCCUCaa-AAAGGCCg--- -3' miRNA: 3'- gCUCUa-CCGGAGaugUUUCCGGacuc -5' |
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13274 | 5' | -53.2 | NC_003409.1 | + | 46985 | 0.67 | 0.924863 |
Target: 5'- gGAGAUuGCCaccgUUUACAAaauauacaggcauuGGGCCUGGGg -3' miRNA: 3'- gCUCUAcCGG----AGAUGUU--------------UCCGGACUC- -5' |
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13274 | 5' | -53.2 | NC_003409.1 | + | 76179 | 0.68 | 0.914997 |
Target: 5'- gGAGAcGGCCUCUA---GGGCCg--- -3' miRNA: 3'- gCUCUaCCGGAGAUguuUCCGGacuc -5' |
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13274 | 5' | -53.2 | NC_003409.1 | + | 77134 | 0.69 | 0.866818 |
Target: 5'- aGAGAUcGGCCUCUGac--GGCCaccGAGa -3' miRNA: 3'- gCUCUA-CCGGAGAUguuuCCGGa--CUC- -5' |
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13274 | 5' | -53.2 | NC_003409.1 | + | 13496 | 0.69 | 0.866818 |
Target: 5'- aGAGAUcucaGGCCUUUGUAGAGGCCa--- -3' miRNA: 3'- gCUCUA----CCGGAGAUGUUUCCGGacuc -5' |
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13274 | 5' | -53.2 | NC_003409.1 | + | 32594 | 0.69 | 0.856604 |
Target: 5'- aCGAGGUGGuCCUgggacgcaagguucCUGCGGAGGUgUGGa -3' miRNA: 3'- -GCUCUACC-GGA--------------GAUGUUUCCGgACUc -5' |
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13274 | 5' | -53.2 | NC_003409.1 | + | 32731 | 0.7 | 0.834238 |
Target: 5'- -aAGGUgGGgCUCUugAGGGGCCUGGc -3' miRNA: 3'- gcUCUA-CCgGAGAugUUUCCGGACUc -5' |
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13274 | 5' | -53.2 | NC_003409.1 | + | 123920 | 0.72 | 0.730256 |
Target: 5'- aCGGGGccuGCCUUUACuccuccaaacugGAAGGCCUGAGa -3' miRNA: 3'- -GCUCUac-CGGAGAUG------------UUUCCGGACUC- -5' |
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13274 | 5' | -53.2 | NC_003409.1 | + | 131832 | 0.72 | 0.689028 |
Target: 5'- uCGAGAUGGCCcuggccggCUGCAGGGGaCUGcAGc -3' miRNA: 3'- -GCUCUACCGGa-------GAUGUUUCCgGAC-UC- -5' |
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13274 | 5' | -53.2 | NC_003409.1 | + | 13665 | 1.1 | 0.003948 |
Target: 5'- gCGAGAUGGCCUCUACAAAGGCCUGAGa -3' miRNA: 3'- -GCUCUACCGGAGAUGUUUCCGGACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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