Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13275 | 3' | -57.9 | NC_003409.1 | + | 17921 | 0.66 | 0.78182 |
Target: 5'- aGGGCggGGUCGCgGGUCggGGcCGCUCc -3' miRNA: 3'- aUUCGaaCCGGCG-CCGGaaCU-GCGAG- -5' |
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13275 | 3' | -57.9 | NC_003409.1 | + | 109823 | 0.66 | 0.78182 |
Target: 5'- gGAGUUUGGuCCGCcGCCcaaGACGC-Cg -3' miRNA: 3'- aUUCGAACC-GGCGcCGGaa-CUGCGaG- -5' |
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13275 | 3' | -57.9 | NC_003409.1 | + | 4178 | 0.66 | 0.761835 |
Target: 5'- gAAGUUUGGCaCGCagagcaGGCCUugcauauUGGCGCcCa -3' miRNA: 3'- aUUCGAACCG-GCG------CCGGA-------ACUGCGaG- -5' |
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13275 | 3' | -57.9 | NC_003409.1 | + | 2800 | 0.66 | 0.743302 |
Target: 5'- gGAGUgUUGGUucuCGCGGUCUUGAgGCa- -3' miRNA: 3'- aUUCG-AACCG---GCGCCGGAACUgCGag -5' |
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13275 | 3' | -57.9 | NC_003409.1 | + | 91940 | 0.67 | 0.733398 |
Target: 5'- cAGGCgacGGCCgGCGGCCcagaaacaggGACGCg- -3' miRNA: 3'- aUUCGaa-CCGG-CGCCGGaa--------CUGCGag -5' |
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13275 | 3' | -57.9 | NC_003409.1 | + | 112955 | 0.67 | 0.717367 |
Target: 5'- --cGCgUGGCCcgcGCGGCCcUUGccaagcgggcgcguuGCGCUCg -3' miRNA: 3'- auuCGaACCGG---CGCCGG-AAC---------------UGCGAG- -5' |
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13275 | 3' | -57.9 | NC_003409.1 | + | 13889 | 0.68 | 0.662073 |
Target: 5'- cGAGCgucaaGGCCGCGGCCaa---GCUUa -3' miRNA: 3'- aUUCGaa---CCGGCGCCGGaacugCGAG- -5' |
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13275 | 3' | -57.9 | NC_003409.1 | + | 55898 | 0.68 | 0.651711 |
Target: 5'- gAAGaggUGGCCGUGcGCCUaGAUGCg- -3' miRNA: 3'- aUUCga-ACCGGCGC-CGGAaCUGCGag -5' |
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13275 | 3' | -57.9 | NC_003409.1 | + | 19450 | 0.69 | 0.589494 |
Target: 5'- ------gGGCCGCuGGCCUUGcCGCUg -3' miRNA: 3'- auucgaaCCGGCG-CCGGAACuGCGAg -5' |
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13275 | 3' | -57.9 | NC_003409.1 | + | 97140 | 0.69 | 0.558711 |
Target: 5'- -cGGUUUauccaGGCCaagGCGGCCggggUGACGCUUg -3' miRNA: 3'- auUCGAA-----CCGG---CGCCGGa---ACUGCGAG- -5' |
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13275 | 3' | -57.9 | NC_003409.1 | + | 116070 | 0.7 | 0.528398 |
Target: 5'- aAAGCUcagGGCaucCGCGGCCgccgGACaGCUCc -3' miRNA: 3'- aUUCGAa--CCG---GCGCCGGaa--CUG-CGAG- -5' |
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13275 | 3' | -57.9 | NC_003409.1 | + | 108445 | 0.7 | 0.514458 |
Target: 5'- cGAGCUagaGGCCGCGGCUacgcacggcgacGACGCUa -3' miRNA: 3'- aUUCGAa--CCGGCGCCGGaa----------CUGCGAg -5' |
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13275 | 3' | -57.9 | NC_003409.1 | + | 20804 | 0.71 | 0.488984 |
Target: 5'- gGAGUUccUGGCCgggcGCGGCCU-GGCGCa- -3' miRNA: 3'- aUUCGA--ACCGG----CGCCGGAaCUGCGag -5' |
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13275 | 3' | -57.9 | NC_003409.1 | + | 109170 | 0.72 | 0.423604 |
Target: 5'- gGGGUUcgUGGCCGcCGGCUggGACGCa- -3' miRNA: 3'- aUUCGA--ACCGGC-GCCGGaaCUGCGag -5' |
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13275 | 3' | -57.9 | NC_003409.1 | + | 14052 | 1.08 | 0.001424 |
Target: 5'- aUAAGCUUGGCCGCGGCCUUGACGCUCg -3' miRNA: 3'- -AUUCGAACCGGCGCCGGAACUGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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