Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13276 | 5' | -54 | NC_003409.1 | + | 11732 | 0.66 | 0.947759 |
Target: 5'- -----cGGCACCGUccucaaGCUGCUCG-GCAg -3' miRNA: 3'- ccgcaaCCGUGGUA------CGACGAGCuUGU- -5' |
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13276 | 5' | -54 | NC_003409.1 | + | 87394 | 0.66 | 0.947759 |
Target: 5'- cGCGggGGCGCUggGCgguugagGUUCGAAgGg -3' miRNA: 3'- cCGCaaCCGUGGuaCGa------CGAGCUUgU- -5' |
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13276 | 5' | -54 | NC_003409.1 | + | 116256 | 0.66 | 0.938456 |
Target: 5'- gGGUGUUGGCAUCGaggaGCUGUcCG-GCGg -3' miRNA: 3'- -CCGCAACCGUGGUa---CGACGaGCuUGU- -5' |
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13276 | 5' | -54 | NC_003409.1 | + | 112930 | 0.67 | 0.910811 |
Target: 5'- aGCG--GGCGCguUGC-GCUCGGGCGg -3' miRNA: 3'- cCGCaaCCGUGguACGaCGAGCUUGU- -5' |
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13276 | 5' | -54 | NC_003409.1 | + | 119807 | 0.67 | 0.90453 |
Target: 5'- cGGCGUgGGCGCCGcGCgcgUGCUCucuCAg -3' miRNA: 3'- -CCGCAaCCGUGGUaCG---ACGAGcuuGU- -5' |
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13276 | 5' | -54 | NC_003409.1 | + | 26938 | 0.68 | 0.876974 |
Target: 5'- gGGCGggGcGCGCCGUGU--UUUGGACAa -3' miRNA: 3'- -CCGCaaC-CGUGGUACGacGAGCUUGU- -5' |
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13276 | 5' | -54 | NC_003409.1 | + | 47279 | 0.68 | 0.861796 |
Target: 5'- cGCGUUaGGCGCag-GCUGUcCGGGCGa -3' miRNA: 3'- cCGCAA-CCGUGguaCGACGaGCUUGU- -5' |
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13276 | 5' | -54 | NC_003409.1 | + | 99493 | 0.69 | 0.820157 |
Target: 5'- uGGCG-UGGCGCagcacaCGUGCUGCgcagCGAGg- -3' miRNA: 3'- -CCGCaACCGUG------GUACGACGa---GCUUgu -5' |
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13276 | 5' | -54 | NC_003409.1 | + | 107817 | 0.72 | 0.687593 |
Target: 5'- cGGCGUUagccagucgaagggcGGCGCCugaaaagGCUGCgcgUCGGGCAg -3' miRNA: 3'- -CCGCAA---------------CCGUGGua-----CGACG---AGCUUGU- -5' |
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13276 | 5' | -54 | NC_003409.1 | + | 23573 | 0.72 | 0.683437 |
Target: 5'- gGGCGUccaauggaaUGGCGCgGUcGCcuaGCUCGAACGg -3' miRNA: 3'- -CCGCA---------ACCGUGgUA-CGa--CGAGCUUGU- -5' |
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13276 | 5' | -54 | NC_003409.1 | + | 119437 | 0.72 | 0.683437 |
Target: 5'- gGGCGUccaauggaaUGGCGCgGUcGCcuaGCUCGAACGg -3' miRNA: 3'- -CCGCA---------ACCGUGgUA-CGa--CGAGCUUGU- -5' |
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13276 | 5' | -54 | NC_003409.1 | + | 107663 | 0.77 | 0.421541 |
Target: 5'- cGGCGUUGGUACUccGCUGC-CaGGGCAu -3' miRNA: 3'- -CCGCAACCGUGGuaCGACGaG-CUUGU- -5' |
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13276 | 5' | -54 | NC_003409.1 | + | 15559 | 1.12 | 0.002475 |
Target: 5'- cGGCGUUGGCACCAUGCUGCUCGAACAg -3' miRNA: 3'- -CCGCAACCGUGGUACGACGAGCUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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