Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13278 | 3' | -51.7 | NC_003409.1 | + | 106148 | 0.66 | 0.987458 |
Target: 5'- uGCGCACGC-UCguggagcacCUGAcccUGGuuGAGGCg -3' miRNA: 3'- -CGCGUGCGcAGaa-------GACU---ACCu-CUUCG- -5' |
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13278 | 3' | -51.7 | NC_003409.1 | + | 4436 | 0.66 | 0.981995 |
Target: 5'- aUGUugGCGUCUccucUCUGAUucuucuGAGGCa -3' miRNA: 3'- cGCGugCGCAGA----AGACUAccu---CUUCG- -5' |
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13278 | 3' | -51.7 | NC_003409.1 | + | 74153 | 0.66 | 0.979599 |
Target: 5'- uGUGCACGCcacggauGUCgccacgUCUGAgGGAccaucgGAGGCa -3' miRNA: 3'- -CGCGUGCG-------CAGa-----AGACUaCCU------CUUCG- -5' |
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13278 | 3' | -51.7 | NC_003409.1 | + | 100019 | 0.67 | 0.971867 |
Target: 5'- cCGCAgacaccugagaccCGCGUCgcUCUGGUGGGacAGCu -3' miRNA: 3'- cGCGU-------------GCGCAGa-AGACUACCUcuUCG- -5' |
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13278 | 3' | -51.7 | NC_003409.1 | + | 104843 | 0.67 | 0.965991 |
Target: 5'- cGCGgGCGCGUUUcgUCUugugauugGGUGGAGucccuGCg -3' miRNA: 3'- -CGCgUGCGCAGA--AGA--------CUACCUCuu---CG- -5' |
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13278 | 3' | -51.7 | NC_003409.1 | + | 6935 | 0.7 | 0.914997 |
Target: 5'- cGUGUGCGUuuuGUCgUCUGAggaGGGGGAGUa -3' miRNA: 3'- -CGCGUGCG---CAGaAGACUa--CCUCUUCG- -5' |
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13278 | 3' | -51.7 | NC_003409.1 | + | 109743 | 0.7 | 0.908852 |
Target: 5'- cGCGCggggaaaaacagACGCG-CUUCUGGcgUGGcGAAGUg -3' miRNA: 3'- -CGCG------------UGCGCaGAAGACU--ACCuCUUCG- -5' |
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13278 | 3' | -51.7 | NC_003409.1 | + | 59013 | 0.71 | 0.866818 |
Target: 5'- gGCGCGgGCucugGUCUUUgcugGGAGggGCg -3' miRNA: 3'- -CGCGUgCG----CAGAAGacuaCCUCuuCG- -5' |
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13278 | 3' | -51.7 | NC_003409.1 | + | 107993 | 0.71 | 0.850951 |
Target: 5'- aUGCGCGCGUacUCUG-UGGcaGGGAGCu -3' miRNA: 3'- cGCGUGCGCAgaAGACuACC--UCUUCG- -5' |
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13278 | 3' | -51.7 | NC_003409.1 | + | 79891 | 0.72 | 0.834238 |
Target: 5'- aGUGCACGCGUC--CUGAuuUGGuGguGCg -3' miRNA: 3'- -CGCGUGCGCAGaaGACU--ACCuCuuCG- -5' |
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13278 | 3' | -51.7 | NC_003409.1 | + | 134660 | 0.72 | 0.825584 |
Target: 5'- aCGCACGCGU----UGGUGGAGgcGUg -3' miRNA: 3'- cGCGUGCGCAgaagACUACCUCuuCG- -5' |
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13278 | 3' | -51.7 | NC_003409.1 | + | 12077 | 0.74 | 0.740362 |
Target: 5'- uCGUACGCGcgcaagauuuuUUUUCUGAccggcucggUGGAGAAGCc -3' miRNA: 3'- cGCGUGCGC-----------AGAAGACU---------ACCUCUUCG- -5' |
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13278 | 3' | -51.7 | NC_003409.1 | + | 22388 | 1.15 | 0.002799 |
Target: 5'- gGCGCACGCGUCUUCUGAUGGAGAAGCa -3' miRNA: 3'- -CGCGUGCGCAGAAGACUACCUCUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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