Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13282 | 5' | -54.3 | NC_003409.1 | + | 97288 | 0.66 | 0.930196 |
Target: 5'- aCUCCAGCuucuCGCaaAGGGCgggGUCCAAa-- -3' miRNA: 3'- gGAGGUCGu---GCG--UCUCG---UAGGUUguu -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 119829 | 0.66 | 0.930196 |
Target: 5'- cUCUCuCAGuCGCGCcuAGCugcuUCCAACAGa -3' miRNA: 3'- -GGAG-GUC-GUGCGucUCGu---AGGUUGUU- -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 99473 | 0.66 | 0.930196 |
Target: 5'- aCUCCAGCugaGCGCAGuauuGGCGUggCgCAGCAc -3' miRNA: 3'- gGAGGUCG---UGCGUC----UCGUA--G-GUUGUu -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 78805 | 0.66 | 0.926391 |
Target: 5'- gCCUCCGGCugGCGcguaacggcaggcguGAgagGCAaacagaaguggguUCCGACAAg -3' miRNA: 3'- -GGAGGUCGugCGU---------------CU---CGU-------------AGGUUGUU- -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 11863 | 0.66 | 0.924722 |
Target: 5'- cCCUCCAgGCugGCuucGGucgcGCAUCCuGCGg -3' miRNA: 3'- -GGAGGU-CGugCG---UCu---CGUAGGuUGUu -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 41769 | 0.66 | 0.918406 |
Target: 5'- -aUCCGGUACGCGccgaaagaccggcGAGCAgCCAugAAa -3' miRNA: 3'- ggAGGUCGUGCGU-------------CUCGUaGGUugUU- -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 130639 | 0.67 | 0.900282 |
Target: 5'- -gUCUGGCugGCAGGGUcucAUCCGcgGCAu -3' miRNA: 3'- ggAGGUCGugCGUCUCG---UAGGU--UGUu -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 57776 | 0.67 | 0.879341 |
Target: 5'- aUUCCGuCuCGCAGGGCGUCCcACAc -3' miRNA: 3'- gGAGGUcGuGCGUCUCGUAGGuUGUu -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 112904 | 0.67 | 0.879341 |
Target: 5'- cCCUCCuGCuCGCAG-GCGggCAGCAc -3' miRNA: 3'- -GGAGGuCGuGCGUCuCGUagGUUGUu -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 41958 | 0.67 | 0.879341 |
Target: 5'- uUCUUCAGCACGguGGGCAgucgcUCUAcguugugaGCGAu -3' miRNA: 3'- -GGAGGUCGUGCguCUCGU-----AGGU--------UGUU- -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 82235 | 0.67 | 0.864197 |
Target: 5'- gCCUCCGGCagACG-AGcGCAUCCGugGu -3' miRNA: 3'- -GGAGGUCG--UGCgUCuCGUAGGUugUu -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 130223 | 0.67 | 0.864196 |
Target: 5'- -aUCCAGCGucgccuuaGCAGAGUGUCCAGUAGa -3' miRNA: 3'- ggAGGUCGUg-------CGUCUCGUAGGUUGUU- -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 64327 | 0.68 | 0.831282 |
Target: 5'- aUCUCCugAGuCACGCugguGAGUAUCCGGCc- -3' miRNA: 3'- -GGAGG--UC-GUGCGu---CUCGUAGGUUGuu -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 51066 | 0.68 | 0.831282 |
Target: 5'- cUCUCCGGCguAUGCAGGagaacGCAUUCGGCGc -3' miRNA: 3'- -GGAGGUCG--UGCGUCU-----CGUAGGUUGUu -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 117691 | 0.68 | 0.831282 |
Target: 5'- --aCCAGCGCGaCGGAaguugGCcUCCAGCAAa -3' miRNA: 3'- ggaGGUCGUGC-GUCU-----CGuAGGUUGUU- -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 61054 | 0.69 | 0.776291 |
Target: 5'- --gCgGGCGCGUGGGGCA-CCAGCAGa -3' miRNA: 3'- ggaGgUCGUGCGUCUCGUaGGUUGUU- -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 107033 | 0.69 | 0.776291 |
Target: 5'- uUUCCAGgGCGUccAGGGCAUCUcGCAGc -3' miRNA: 3'- gGAGGUCgUGCG--UCUCGUAGGuUGUU- -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 37255 | 0.69 | 0.776291 |
Target: 5'- aUCgCCAGCAUGCaAGGGCcgCCAAgAAg -3' miRNA: 3'- -GGaGGUCGUGCG-UCUCGuaGGUUgUU- -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 63455 | 0.7 | 0.756755 |
Target: 5'- uCCUCCAGCuuGCAGGaCAUCgCGGCc- -3' miRNA: 3'- -GGAGGUCGugCGUCUcGUAG-GUUGuu -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 4164 | 0.7 | 0.730667 |
Target: 5'- gCCUUCgugcacuagaaguuuGGCACGCAGAGCAggCCuuGCAu -3' miRNA: 3'- -GGAGG---------------UCGUGCGUCUCGUa-GGu-UGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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