Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13283 | 5' | -56.5 | NC_003409.1 | + | 6397 | 0.66 | 0.88688 |
Target: 5'- cGgGGGUGCCAUggACGGccgaggguguggccUCGGUcaUCCAGg -3' miRNA: 3'- -CgUCCGCGGUG--UGCC--------------AGUCA--AGGUCg -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 120215 | 0.66 | 0.882014 |
Target: 5'- cCAGGCGgcuggCACACGGgccgugAGcgCCAGCa -3' miRNA: 3'- cGUCCGCg----GUGUGCCag----UCaaGGUCG- -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 39697 | 0.66 | 0.882014 |
Target: 5'- aCAGGUGUUguaGC-CGGuUCAGUUUUAGCu -3' miRNA: 3'- cGUCCGCGG---UGuGCC-AGUCAAGGUCG- -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 95553 | 0.66 | 0.882014 |
Target: 5'- aGUuGGCGCCGCugGGgggCAG--CUGGUg -3' miRNA: 3'- -CGuCCGCGGUGugCCa--GUCaaGGUCG- -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 80282 | 0.66 | 0.867518 |
Target: 5'- --uGGCGCCcuACACGGggAGUuucgugaccacgUCCAGUc -3' miRNA: 3'- cguCCGCGG--UGUGCCagUCA------------AGGUCG- -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 42600 | 0.66 | 0.867518 |
Target: 5'- uGCAGGUGCgAguUGGUggcCAGacagcagaauauUUCCAGCu -3' miRNA: 3'- -CGUCCGCGgUguGCCA---GUC------------AAGGUCG- -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 80483 | 0.66 | 0.867518 |
Target: 5'- aCAGGUGCCGCACcugaGGUUgcaaaacgAGUUCgCuGGCa -3' miRNA: 3'- cGUCCGCGGUGUG----CCAG--------UCAAG-G-UCG- -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 90150 | 0.66 | 0.867518 |
Target: 5'- gGCcGGCGUCugGacgcCGGcggCAG-UCCAGCa -3' miRNA: 3'- -CGuCCGCGGugU----GCCa--GUCaAGGUCG- -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 103638 | 0.66 | 0.859948 |
Target: 5'- aGgGGGCGgCugGCgGGUCGGgugUCCugacGCa -3' miRNA: 3'- -CgUCCGCgGugUG-CCAGUCa--AGGu---CG- -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 8097 | 0.66 | 0.852171 |
Target: 5'- gGCAGGa-CCugACGGUCGacugUCCAacGCg -3' miRNA: 3'- -CGUCCgcGGugUGCCAGUca--AGGU--CG- -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 16790 | 0.67 | 0.836025 |
Target: 5'- --uGGCgGCCGCGCGGgg----CCAGCg -3' miRNA: 3'- cguCCG-CGGUGUGCCagucaaGGUCG- -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 36914 | 0.67 | 0.819135 |
Target: 5'- cCAGGCGCaCGCuGCGGaagCAGauUUCCGGa -3' miRNA: 3'- cGUCCGCG-GUG-UGCCa--GUC--AAGGUCg -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 56061 | 0.67 | 0.81043 |
Target: 5'- -uGGGCGuCCACAUGG-CAGcugaaUCAGCa -3' miRNA: 3'- cgUCCGC-GGUGUGCCaGUCaa---GGUCG- -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 32111 | 0.67 | 0.81043 |
Target: 5'- -gGGGCGCCgACGCGGcaCAGUggCCGuGUa -3' miRNA: 3'- cgUCCGCGG-UGUGCCa-GUCAa-GGU-CG- -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 82625 | 0.68 | 0.783374 |
Target: 5'- uGUAGGCGUCGCGUGGUCuGcgCCGa- -3' miRNA: 3'- -CGUCCGCGGUGUGCCAGuCaaGGUcg -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 16633 | 0.68 | 0.764641 |
Target: 5'- cGCuGGCcCCGCGCGGccgcCAGguagUCUGGCa -3' miRNA: 3'- -CGuCCGcGGUGUGCCa---GUCa---AGGUCG- -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 19153 | 0.68 | 0.755093 |
Target: 5'- aGgAGGgGCC-CGCGGgggCAGcUCCAGg -3' miRNA: 3'- -CgUCCgCGGuGUGCCa--GUCaAGGUCg -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 8750 | 0.68 | 0.755093 |
Target: 5'- cGCAGGCGCgGCGCacucgaGGggugacaccuuUCAGacgUCCAGUu -3' miRNA: 3'- -CGUCCGCGgUGUG------CC-----------AGUCa--AGGUCG- -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 120386 | 0.69 | 0.745436 |
Target: 5'- uGCuGGCGCU-CACGGcCcGUgugCCAGCc -3' miRNA: 3'- -CGuCCGCGGuGUGCCaGuCAa--GGUCG- -5' |
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13283 | 5' | -56.5 | NC_003409.1 | + | 74626 | 0.69 | 0.725831 |
Target: 5'- aGCGGGCGUCGCcgaGGUCgcccugaccGGUUgCAGUu -3' miRNA: 3'- -CGUCCGCGGUGug-CCAG---------UCAAgGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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