Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13283 | 5' | -56.5 | NC_003409.1 | + | 80282 | 0.66 | 0.867518 |
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Target: 5'- --uGGCGCCcuACACGGggAGUuucgugaccacgUCCAGUc -3' miRNA: 3'- cguCCGCGG--UGUGCCagUCA------------AGGUCG- -5' |
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| 13283 | 5' | -56.5 | NC_003409.1 | + | 42600 | 0.66 | 0.867518 |
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Target: 5'- uGCAGGUGCgAguUGGUggcCAGacagcagaauauUUCCAGCu -3' miRNA: 3'- -CGUCCGCGgUguGCCA---GUC------------AAGGUCG- -5' |
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| 13283 | 5' | -56.5 | NC_003409.1 | + | 80483 | 0.66 | 0.867518 |
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Target: 5'- aCAGGUGCCGCACcugaGGUUgcaaaacgAGUUCgCuGGCa -3' miRNA: 3'- cGUCCGCGGUGUG----CCAG--------UCAAG-G-UCG- -5' |
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| 13283 | 5' | -56.5 | NC_003409.1 | + | 39697 | 0.66 | 0.882014 |
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Target: 5'- aCAGGUGUUguaGC-CGGuUCAGUUUUAGCu -3' miRNA: 3'- cGUCCGCGG---UGuGCC-AGUCAAGGUCG- -5' |
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| 13283 | 5' | -56.5 | NC_003409.1 | + | 95553 | 0.66 | 0.882014 |
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Target: 5'- aGUuGGCGCCGCugGGgggCAG--CUGGUg -3' miRNA: 3'- -CGuCCGCGGUGugCCa--GUCaaGGUCG- -5' |
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| 13283 | 5' | -56.5 | NC_003409.1 | + | 120215 | 0.66 | 0.882014 |
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Target: 5'- cCAGGCGgcuggCACACGGgccgugAGcgCCAGCa -3' miRNA: 3'- cGUCCGCg----GUGUGCCag----UCaaGGUCG- -5' |
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| 13283 | 5' | -56.5 | NC_003409.1 | + | 6397 | 0.66 | 0.88688 |
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Target: 5'- cGgGGGUGCCAUggACGGccgaggguguggccUCGGUcaUCCAGg -3' miRNA: 3'- -CgUCCGCGGUG--UGCC--------------AGUCA--AGGUCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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