Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13285 | 3' | -46.7 | NC_003409.1 | + | 122993 | 0.66 | 0.999898 |
Target: 5'- --aUCAGCGCugcagGCUGUGCGCUg -3' miRNA: 3'- caaAGUUGUGcauagUGAUACGCGGg -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 41744 | 0.66 | 0.999898 |
Target: 5'- ---aCAugACGaGUCugUagauggccgGUGUGCCCg -3' miRNA: 3'- caaaGUugUGCaUAGugA---------UACGCGGG- -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 22341 | 0.66 | 0.999867 |
Target: 5'- ----aAGCGCGUAgCGCUucGCGCCg -3' miRNA: 3'- caaagUUGUGCAUaGUGAuaCGCGGg -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 34100 | 0.66 | 0.999867 |
Target: 5'- ----gAGCGCGccUCGCUG-GCGCCUc -3' miRNA: 3'- caaagUUGUGCauAGUGAUaCGCGGG- -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 81563 | 0.66 | 0.999827 |
Target: 5'- ---cCGACAUGaccggCGCUGUG-GCCCg -3' miRNA: 3'- caaaGUUGUGCaua--GUGAUACgCGGG- -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 102479 | 0.66 | 0.999827 |
Target: 5'- ---gCAGCGCGUGUaCGCcgcGCuGCCCu -3' miRNA: 3'- caaaGUUGUGCAUA-GUGauaCG-CGGG- -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 119968 | 0.66 | 0.999776 |
Target: 5'- ----gAGCACGcg-CGCg--GCGCCCa -3' miRNA: 3'- caaagUUGUGCauaGUGauaCGCGGG- -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 26930 | 0.67 | 0.999713 |
Target: 5'- ---aCGACagGCGUuUCugUGUGCGCUUc -3' miRNA: 3'- caaaGUUG--UGCAuAGugAUACGCGGG- -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 82664 | 0.67 | 0.999635 |
Target: 5'- ---gCAGCGCGa--CACUGUcCGCCCc -3' miRNA: 3'- caaaGUUGUGCauaGUGAUAcGCGGG- -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 68283 | 0.67 | 0.999422 |
Target: 5'- uUUUCAACcuuuaucUCACU-UGCGCCCg -3' miRNA: 3'- cAAAGUUGugcau--AGUGAuACGCGGG- -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 60730 | 0.68 | 0.999109 |
Target: 5'- --aUCA-CAUcauUCACUAUGCGaCCCa -3' miRNA: 3'- caaAGUuGUGcauAGUGAUACGC-GGG- -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 33817 | 0.68 | 0.998882 |
Target: 5'- ---cCAugGCGgg-CGCUGUcccuggcGCGCCCg -3' miRNA: 3'- caaaGUugUGCauaGUGAUA-------CGCGGG- -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 99653 | 0.68 | 0.998836 |
Target: 5'- ---gCAGCACGUGU-GCUGcgccacgccaauacUGCGCUCa -3' miRNA: 3'- caaaGUUGUGCAUAgUGAU--------------ACGCGGG- -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 45908 | 0.68 | 0.998661 |
Target: 5'- --cUCAcCAUGg--CGCUAaugGCGCCCg -3' miRNA: 3'- caaAGUuGUGCauaGUGAUa--CGCGGG- -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 21755 | 0.7 | 0.994523 |
Target: 5'- uUUUUGACaaaGCGUGUCGCcaggUGUGUGCCg -3' miRNA: 3'- cAAAGUUG---UGCAUAGUG----AUACGCGGg -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 30079 | 0.7 | 0.991435 |
Target: 5'- --cUguGCGCGU---GCUAUGUGCCCu -3' miRNA: 3'- caaAguUGUGCAuagUGAUACGCGGG- -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 113424 | 0.7 | 0.991435 |
Target: 5'- ---gCAACACGgcgAUCGCc-UGCGCCa -3' miRNA: 3'- caaaGUUGUGCa--UAGUGauACGCGGg -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 25895 | 0.71 | 0.985347 |
Target: 5'- ---aCAGCGCGUAcCGacCUAUGCGCgCCc -3' miRNA: 3'- caaaGUUGUGCAUaGU--GAUACGCG-GG- -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 115972 | 0.73 | 0.970667 |
Target: 5'- -aUUCAcaaGCACcUGUucugcgaccCGCUGUGCGCCCu -3' miRNA: 3'- caAAGU---UGUGcAUA---------GUGAUACGCGGG- -5' |
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13285 | 3' | -46.7 | NC_003409.1 | + | 31930 | 0.73 | 0.956323 |
Target: 5'- --cUCGGCGCGUGUCcguacacggccACUGUgccgcgucgGCGCCCc -3' miRNA: 3'- caaAGUUGUGCAUAG-----------UGAUA---------CGCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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