Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13285 | 5' | -57.7 | NC_003409.1 | + | 32536 | 0.66 | 0.869219 |
Target: 5'- cCGA-GCauGGCACAG-GGCC-UGUACGu -3' miRNA: 3'- -GCUgCG--CCGUGUCaCCGGcACAUGCc -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 113590 | 0.66 | 0.86186 |
Target: 5'- cCGuCGCuGGCGCAGgcgaucGCCGUGuUGCGc -3' miRNA: 3'- -GCuGCG-CCGUGUCac----CGGCAC-AUGCc -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 86602 | 0.66 | 0.853536 |
Target: 5'- uGACGCgGGCGaggcaauguuaugUAGcUGGCC-UGUugGGg -3' miRNA: 3'- gCUGCG-CCGU-------------GUC-ACCGGcACAugCC- -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 118860 | 0.66 | 0.846551 |
Target: 5'- uGGCuaacaCGGCugGaGUGGCgGUGgGCGGg -3' miRNA: 3'- gCUGc----GCCGugU-CACCGgCACaUGCC- -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 110047 | 0.66 | 0.846551 |
Target: 5'- gGugGCaaggccguaggGGUACGGUGGCaGUGUugcgcCGGg -3' miRNA: 3'- gCugCG-----------CCGUGUCACCGgCACAu----GCC- -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 100880 | 0.66 | 0.84181 |
Target: 5'- gGAaGCGGCaaaaGCgccacagacucacaaAGUGGCCGgugugugGUACGGa -3' miRNA: 3'- gCUgCGCCG----UG---------------UCACCGGCa------CAUGCC- -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 24211 | 0.66 | 0.838613 |
Target: 5'- --cUGCGcGCGCGGcGGCaCGUGggACGGa -3' miRNA: 3'- gcuGCGC-CGUGUCaCCG-GCACa-UGCC- -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 45464 | 0.66 | 0.836196 |
Target: 5'- gGAUGUGGCGCAGaccgugcuuguuaaUGGCUuugGUGCGu -3' miRNA: 3'- gCUGCGCCGUGUC--------------ACCGGca-CAUGCc -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 59967 | 0.67 | 0.805127 |
Target: 5'- uGucuCGCGGC--GGUGGCCGUaUAuCGGg -3' miRNA: 3'- gCu--GCGCCGugUCACCGGCAcAU-GCC- -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 110969 | 0.67 | 0.805127 |
Target: 5'- aGAUGCGGUAUAGgcgacGGgCGaUGUGCuGGg -3' miRNA: 3'- gCUGCGCCGUGUCa----CCgGC-ACAUG-CC- -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 81557 | 0.67 | 0.78745 |
Target: 5'- uGAC-CGGCGCuGUGGCCcggggaGUG-AUGGa -3' miRNA: 3'- gCUGcGCCGUGuCACCGG------CACaUGCC- -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 120208 | 0.68 | 0.759948 |
Target: 5'- uCGACGUccaggcggcuGGCACAcgGGCCGUGaGCGc -3' miRNA: 3'- -GCUGCG----------CCGUGUcaCCGGCACaUGCc -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 88023 | 0.68 | 0.759948 |
Target: 5'- cCGGCGUcGCACAGacGCCGgggGaUACGGg -3' miRNA: 3'- -GCUGCGcCGUGUCacCGGCa--C-AUGCC- -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 45870 | 0.68 | 0.750551 |
Target: 5'- aGACGCGGCAUAc--GCCGcg-ACGGg -3' miRNA: 3'- gCUGCGCCGUGUcacCGGCacaUGCC- -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 87520 | 0.68 | 0.731467 |
Target: 5'- -cGCGCGaGCACAuUGGCCGcuUugGGg -3' miRNA: 3'- gcUGCGC-CGUGUcACCGGCacAugCC- -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 40537 | 0.69 | 0.682459 |
Target: 5'- gGACGCGGCcguuCGGUGGCgCcaGUGCaGGc -3' miRNA: 3'- gCUGCGCCGu---GUCACCG-GcaCAUG-CC- -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 127543 | 0.7 | 0.622414 |
Target: 5'- gCGGCGCGuGCcauCGGUGGCCaucgGCGGu -3' miRNA: 3'- -GCUGCGC-CGu--GUCACCGGcacaUGCC- -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 88705 | 0.7 | 0.612387 |
Target: 5'- -uGCGUGGaacCAGgagGGCCGUcGUGCGGa -3' miRNA: 3'- gcUGCGCCgu-GUCa--CCGGCA-CAUGCC- -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 25940 | 0.72 | 0.49499 |
Target: 5'- cCGGCGCGGCGuCGGUauGGCa--GUACGGu -3' miRNA: 3'- -GCUGCGCCGU-GUCA--CCGgcaCAUGCC- -5' |
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13285 | 5' | -57.7 | NC_003409.1 | + | 54409 | 0.73 | 0.467139 |
Target: 5'- gCGugGCGGgAUGGU-GCCGUGUGCa- -3' miRNA: 3'- -GCugCGCCgUGUCAcCGGCACAUGcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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