Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13286 | 3' | -63.5 | NC_003409.1 | + | 103419 | 0.66 | 0.548287 |
Target: 5'- gGCCUCCCugGUGGCCuGuucgauGGUCUgUa -3' miRNA: 3'- gUGGAGGGugCACCGGuC------UCGGGgGc -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 111956 | 0.66 | 0.548287 |
Target: 5'- uGCUUCCCGagacccccaGUGaGUCcuGGCCCCCGg -3' miRNA: 3'- gUGGAGGGUg--------CAC-CGGucUCGGGGGC- -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 55842 | 0.66 | 0.538746 |
Target: 5'- gACCUCCCACG-GGUCGccgacauuGAcGCCCUgGu -3' miRNA: 3'- gUGGAGGGUGCaCCGGU--------CU-CGGGGgC- -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 7630 | 0.66 | 0.519842 |
Target: 5'- gGCaUCCgA-GUGGCCAGAGCUCCa- -3' miRNA: 3'- gUGgAGGgUgCACCGGUCUCGGGGgc -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 11010 | 0.66 | 0.519842 |
Target: 5'- gCACCUCCUA-GcGG-CGGAGCCCCa- -3' miRNA: 3'- -GUGGAGGGUgCaCCgGUCUCGGGGgc -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 20627 | 0.67 | 0.482865 |
Target: 5'- -cCCUCCCuucUGUGcGCCAGGccGCgCCCGg -3' miRNA: 3'- guGGAGGGu--GCAC-CGGUCU--CGgGGGC- -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 118001 | 0.67 | 0.473818 |
Target: 5'- -cCC-CCCGaGUGGCCAGcguGGCCCCg- -3' miRNA: 3'- guGGaGGGUgCACCGGUC---UCGGGGgc -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 113026 | 0.67 | 0.473818 |
Target: 5'- gGCCUCCCG-GaGGCCGGuaucugccGCCCCUa -3' miRNA: 3'- gUGGAGGGUgCaCCGGUCu-------CGGGGGc -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 79784 | 0.68 | 0.455102 |
Target: 5'- aCGCCUCCa--GUGGCCGGccGCcaaggaaCCCCGa -3' miRNA: 3'- -GUGGAGGgugCACCGGUCu-CG-------GGGGC- -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 85352 | 0.68 | 0.438519 |
Target: 5'- uCGCCUCUCuauaucUGaUGGCCGGuGGCuCCCCGg -3' miRNA: 3'- -GUGGAGGGu-----GC-ACCGGUC-UCG-GGGGC- -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 112008 | 0.68 | 0.438519 |
Target: 5'- cCGCC-CCCGCgGUGGCCGacgcGAGgaaaCCCCCc -3' miRNA: 3'- -GUGGaGGGUG-CACCGGU----CUC----GGGGGc -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 112963 | 0.68 | 0.413055 |
Target: 5'- uCugUUCUCGCGUGGCCcGcgcGGCCCuuGc -3' miRNA: 3'- -GugGAGGGUGCACCGGuC---UCGGGggC- -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 125466 | 0.68 | 0.413055 |
Target: 5'- uGgCUCCCGCugcuGUGGCUcccgcugcuGGGGCUCCCGc -3' miRNA: 3'- gUgGAGGGUG----CACCGG---------UCUCGGGGGC- -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 44174 | 0.68 | 0.408073 |
Target: 5'- aACCaCCCACGUcucggucgacuggauGGCCGGGgauucuacGCCCCUGa -3' miRNA: 3'- gUGGaGGGUGCA---------------CCGGUCU--------CGGGGGC- -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 18964 | 0.69 | 0.371932 |
Target: 5'- uGCC-CCCGCGgGGCCauuuuggucgccuGGAgcuGCCCCCGc -3' miRNA: 3'- gUGGaGGGUGCaCCGG-------------UCU---CGGGGGC- -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 133772 | 0.7 | 0.34242 |
Target: 5'- gGCCUagCCACG-GGCUGGGGaCCCUCGg -3' miRNA: 3'- gUGGAg-GGUGCaCCGGUCUC-GGGGGC- -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 109316 | 0.7 | 0.327955 |
Target: 5'- gCGCCgucuugggCCCACGgGaGCCGGAGCacgccgCCCCGg -3' miRNA: 3'- -GUGGa-------GGGUGCaC-CGGUCUCG------GGGGC- -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 24111 | 0.71 | 0.268509 |
Target: 5'- cCACCgaacaaCCC-CGUGGaCAGGGCCCCUa -3' miRNA: 3'- -GUGGa-----GGGuGCACCgGUCUCGGGGGc -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 42029 | 0.74 | 0.18912 |
Target: 5'- cCACC-CCCACGUGGCCAuGaAGCUgCUGa -3' miRNA: 3'- -GUGGaGGGUGCACCGGU-C-UCGGgGGC- -5' |
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13286 | 3' | -63.5 | NC_003409.1 | + | 19277 | 0.74 | 0.171747 |
Target: 5'- aGCCaaaCCCACGUcgcagcggcaaGGCCAGcGGCCCCCa -3' miRNA: 3'- gUGGa--GGGUGCA-----------CCGGUC-UCGGGGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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