Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13286 | 5' | -48.6 | NC_003409.1 | + | 36603 | 0.66 | 0.999061 |
Target: 5'- ---gAGGGAacACCACUGcUGGGGAg-- -3' miRNA: 3'- gggaUUCCU--UGGUGACaACCUCUaug -5' |
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13286 | 5' | -48.6 | NC_003409.1 | + | 32241 | 1.12 | 0.00837 |
Target: 5'- uCCCUAAGGAACCACUGUUGGAGAUACu -3' miRNA: 3'- -GGGAUUCCUUGGUGACAACCUCUAUG- -5' |
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13286 | 5' | -48.6 | NC_003409.1 | + | 32058 | 0.68 | 0.99353 |
Target: 5'- cCCC--GGGAGCCACgugUGGAG-UAUc -3' miRNA: 3'- -GGGauUCCUUGGUGacaACCUCuAUG- -5' |
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13286 | 5' | -48.6 | NC_003409.1 | + | 15377 | 0.67 | 0.997094 |
Target: 5'- aCCCUGAGGAuucaAUUGCUGUUcGAGcaGCa -3' miRNA: 3'- -GGGAUUCCU----UGGUGACAAcCUCuaUG- -5' |
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13286 | 5' | -48.6 | NC_003409.1 | + | 8715 | 0.66 | 0.998851 |
Target: 5'- aCCCUGGGGAcugucAUC-CUGUUGGuc-UGCu -3' miRNA: 3'- -GGGAUUCCU-----UGGuGACAACCucuAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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