Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13288 | 3' | -58.4 | NC_003409.1 | + | 100973 | 0.66 | 0.821892 |
Target: 5'- -aAUCCCgCACCC-CCCUggcaaaCUCCGGu- -3' miRNA: 3'- ggUAGGG-GUGGGuGGGAa-----GAGGUCcu -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 34553 | 0.66 | 0.803859 |
Target: 5'- aUCGUCCCCggggACCCucaCUUCUCCguugacaGGGAc -3' miRNA: 3'- -GGUAGGGG----UGGGuggGAAGAGG-------UCCU- -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 121859 | 0.66 | 0.795922 |
Target: 5'- cCCAcCCCCAcauccuuccuCCCACCCUg--CCAGu- -3' miRNA: 3'- -GGUaGGGGU----------GGGUGGGAagaGGUCcu -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 112144 | 0.66 | 0.786968 |
Target: 5'- gCAUCCggCACCaCGCCCgggggCCAGGAc -3' miRNA: 3'- gGUAGGg-GUGG-GUGGGaaga-GGUCCU- -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 26792 | 0.66 | 0.786968 |
Target: 5'- gCG-CCCCGCCCuuCCUUCccUCCGGa- -3' miRNA: 3'- gGUaGGGGUGGGugGGAAG--AGGUCcu -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 71042 | 0.67 | 0.768663 |
Target: 5'- gCCAUCUCUGCCCcCCCaagCUCUuugucccacGGGAg -3' miRNA: 3'- -GGUAGGGGUGGGuGGGaa-GAGG---------UCCU- -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 55947 | 0.67 | 0.759329 |
Target: 5'- ---cCCCCACUCACUCcUCUUCGGaGAu -3' miRNA: 3'- gguaGGGGUGGGUGGGaAGAGGUC-CU- -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 68848 | 0.67 | 0.759329 |
Target: 5'- gCCAagUCCCUcguggagccgGCCCucCCCUUCUCCAc-- -3' miRNA: 3'- -GGU--AGGGG----------UGGGu-GGGAAGAGGUccu -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 105481 | 0.67 | 0.749887 |
Target: 5'- -aGUCCCCGuCCgCugCUUUCUCgGGGu -3' miRNA: 3'- ggUAGGGGU-GG-GugGGAAGAGgUCCu -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 93788 | 0.67 | 0.720992 |
Target: 5'- gCCGUCCaaagcaGCCCagGCCaguucagUCUCCGGGAu -3' miRNA: 3'- -GGUAGGgg----UGGG--UGGga-----AGAGGUCCU- -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 67852 | 0.68 | 0.7112 |
Target: 5'- gCCAuuuUUCCCACcagCCACCCUcgUCCgAGGAg -3' miRNA: 3'- -GGU---AGGGGUG---GGUGGGAagAGG-UCCU- -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 66499 | 0.68 | 0.695403 |
Target: 5'- aCCAUcCCCCACCCACgcggcucuuCCaacacugguggccuaUUaCUCCGGGGc -3' miRNA: 3'- -GGUA-GGGGUGGGUG---------GG---------------AA-GAGGUCCU- -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 84787 | 0.68 | 0.691431 |
Target: 5'- uUCGUCUu--CCCACUCUaCUCCGGGAa -3' miRNA: 3'- -GGUAGGgguGGGUGGGAaGAGGUCCU- -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 119045 | 0.69 | 0.65943 |
Target: 5'- gCCAggauuauaggggCCCCGCCCACCgCcaCUCCAGc- -3' miRNA: 3'- -GGUa-----------GGGGUGGGUGG-GaaGAGGUCcu -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 68549 | 0.69 | 0.650379 |
Target: 5'- aUAUUCCCACcuCCACCuCUUUcaucacuUCCAGGAu -3' miRNA: 3'- gGUAGGGGUG--GGUGG-GAAG-------AGGUCCU- -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 126911 | 0.7 | 0.601047 |
Target: 5'- gCAUUgCCACCCACgCagUCUCCAGa- -3' miRNA: 3'- gGUAGgGGUGGGUGgGa-AGAGGUCcu -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 98109 | 0.7 | 0.561129 |
Target: 5'- aCGUCCCCACCauacugucuaacCACCCgaaggUCgUCgGGGAg -3' miRNA: 3'- gGUAGGGGUGG------------GUGGGa----AG-AGgUCCU- -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 111538 | 0.71 | 0.531669 |
Target: 5'- -uGUCCCaACCCGCCUUuaUCUCCGGcGGg -3' miRNA: 3'- ggUAGGGgUGGGUGGGA--AGAGGUC-CU- -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 36547 | 0.72 | 0.465393 |
Target: 5'- cCCcUCCCagACCCACCCccaacgUUCCAGGGc -3' miRNA: 3'- -GGuAGGGg-UGGGUGGGaa----GAGGUCCU- -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 90810 | 0.73 | 0.40367 |
Target: 5'- cCCA-CCCCuCCCACUCUUUccCCAGGGg -3' miRNA: 3'- -GGUaGGGGuGGGUGGGAAGa-GGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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