Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13288 | 3' | -58.4 | NC_003409.1 | + | 119045 | 0.69 | 0.65943 |
Target: 5'- gCCAggauuauaggggCCCCGCCCACCgCcaCUCCAGc- -3' miRNA: 3'- -GGUa-----------GGGGUGGGUGG-GaaGAGGUCcu -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 121859 | 0.66 | 0.795922 |
Target: 5'- cCCAcCCCCAcauccuuccuCCCACCCUg--CCAGu- -3' miRNA: 3'- -GGUaGGGGU----------GGGUGGGAagaGGUCcu -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 126911 | 0.7 | 0.601047 |
Target: 5'- gCAUUgCCACCCACgCagUCUCCAGa- -3' miRNA: 3'- gGUAGgGGUGGGUGgGa-AGAGGUCcu -5' |
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13288 | 3' | -58.4 | NC_003409.1 | + | 126972 | 0.76 | 0.290207 |
Target: 5'- cCCaAUCCCUccauCCCAUCCUgugUCUCCGGGGa -3' miRNA: 3'- -GG-UAGGGGu---GGGUGGGA---AGAGGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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