Results 61 - 64 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
13291 | 3' | -52.5 | NC_003409.1 | + | 107028 | 0.66 | 0.954386 |
Target: 5'- aGGGCgUCCAG-GGCAucuCGCAGcAGGGUu -3' miRNA: 3'- -UCCGaAGGUCgUCGU---GCGUUuUCUCG- -5' |
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13291 | 3' | -52.5 | NC_003409.1 | + | 107800 | 0.66 | 0.953974 |
Target: 5'- aGGGCggcgCCugaaaaGGCuGCGCGUcgggcagAAAGGAGCg -3' miRNA: 3'- -UCCGaa--GG------UCGuCGUGCG-------UUUUCUCG- -5' |
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13291 | 3' | -52.5 | NC_003409.1 | + | 103019 | 0.66 | 0.968864 |
Target: 5'- uAGGCUcuggCUAGCGGgcgcCugGCAGGgauGAGCc -3' miRNA: 3'- -UCCGAa---GGUCGUC----GugCGUUUu--CUCG- -5' |
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13291 | 3' | -52.5 | NC_003409.1 | + | 60122 | 0.66 | 0.968864 |
Target: 5'- cGGuCUUUUuaacuuucgccaGGCGGCGCugcgGCGGGAGAGCc -3' miRNA: 3'- uCC-GAAGG------------UCGUCGUG----CGUUUUCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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