miRNA: 3'- -UCCgaa-GGUCGUCGUGcGuuUUCUCG- -5'
miRNA: 3'- -UCCgaa-GGUCGUCGUGcGuuUUCUCG- -5'
miRNA: 3'- -UCCgaa-GGUCGUCGUGcGuuUUCUCG- -5'
miRNA: 3'- -UCCGaaGGUCG--UCGUGCGuu--UUCUCG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13291 | 3' | -52.5 | NC_003409.1 | + | 24300 | 0.67 | 0.935927 |
|
Target: 5'- cAGGagccCCGGCAGCAC-CccAGGAGCc -3' miRNA: 3'- -UCCgaa-GGUCGUCGUGcGuuUUCUCG- -5' |
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| 13291 | 3' | -52.5 | NC_003409.1 | + | 24340 | 0.67 | 0.935927 |
|
Target: 5'- cAGGagccCCGGCAGCAC-CccAGGAGCc -3' miRNA: 3'- -UCCgaa-GGUCGUCGUGcGuuUUCUCG- -5' |
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| 13291 | 3' | -52.5 | NC_003409.1 | + | 24380 | 0.67 | 0.935927 |
|
Target: 5'- cAGGagccCCGGCAGCAC-CccAGGAGCc -3' miRNA: 3'- -UCCgaa-GGUCGUCGUGcGuuUUCUCG- -5' |
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| 13291 | 3' | -52.5 | NC_003409.1 | + | 33942 | 0.71 | 0.764717 |
|
Target: 5'- gAGGCg-CCAGCgaGGCGCGCuccuGGGGGUu -3' miRNA: 3'- -UCCGaaGGUCG--UCGUGCGuu--UUCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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