Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 133 | 5' | -60 | AC_000006.1 | + | 10822 | 0.66 | 0.341964 |
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Target: 5'- cAGcUGCacgCgGGGgUGUCGCUCCGGGGa -3' miRNA: 3'- -UCuACGa--GgUCCaGCAGCGGGGCCUC- -5' |
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| 133 | 5' | -60 | AC_000006.1 | + | 4620 | 0.66 | 0.333799 |
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Target: 5'- gAGAUGCagaUCUgcgAGGggaaCuUCGCCCCGGGGc -3' miRNA: 3'- -UCUACG---AGG---UCCa---GcAGCGGGGCCUC- -5' |
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| 133 | 5' | -60 | AC_000006.1 | + | 22648 | 0.69 | 0.217301 |
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Target: 5'- -cGUGCUCCGGcGgauagcgcgccgaccCGUgGCCCCGGGGc -3' miRNA: 3'- ucUACGAGGUC-Ca--------------GCAgCGGGGCCUC- -5' |
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| 133 | 5' | -60 | AC_000006.1 | + | 23058 | 0.74 | 0.092126 |
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Target: 5'- gGGGUuucGCUCC-GGUCGgccagggCGUCCCGGAGg -3' miRNA: 3'- -UCUA---CGAGGuCCAGCa------GCGGGGCCUC- -5' |
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| 133 | 5' | -60 | AC_000006.1 | + | 15706 | 1.09 | 0.00021 |
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Target: 5'- gAGAUGCUCCAGGUCGUCGCCCCGGAGa -3' miRNA: 3'- -UCUACGAGGUCCAGCAGCGGGGCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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