Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13307 | 3' | -58.5 | NC_003409.1 | + | 133128 | 0.66 | 0.760082 |
Target: 5'- gUGCGCACccaccugccugcggaUC-CCGCGGCCGGUCc- -3' miRNA: 3'- uGCGCGUGc--------------AGaGGUGCCGGCUAGuu -5' |
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13307 | 3' | -58.5 | NC_003409.1 | + | 67714 | 0.67 | 0.73016 |
Target: 5'- -gGCGCGCGUCgguaaaCGCGGCC--UCAu -3' miRNA: 3'- ugCGCGUGCAGag----GUGCCGGcuAGUu -5' |
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13307 | 3' | -58.5 | NC_003409.1 | + | 133436 | 0.68 | 0.649854 |
Target: 5'- gUGCGCACGgggUC-CCAgGGCCGGUUc- -3' miRNA: 3'- uGCGCGUGC---AGaGGUgCCGGCUAGuu -5' |
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13307 | 3' | -58.5 | NC_003409.1 | + | 11882 | 0.69 | 0.609088 |
Target: 5'- uCGCGCauccuGCGgcuUCUCCAcCGaGCCGGUCAGa -3' miRNA: 3'- uGCGCG-----UGC---AGAGGU-GC-CGGCUAGUU- -5' |
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13307 | 3' | -58.5 | NC_003409.1 | + | 54429 | 1.05 | 0.00256 |
Target: 5'- gACGCGCACGUCUCCACGGCCGAUCAAc -3' miRNA: 3'- -UGCGCGUGCAGAGGUGCCGGCUAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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