miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
13309 5' -56.9 NC_003409.1 + 122041 0.66 0.82531
Target:  5'- gAAGCGGGUUgGacuGGCAGGGUGGgaGg -3'
miRNA:   3'- -UUCGCCCGGgCg--CUGUUUCACCaaCa -5'
13309 5' -56.9 NC_003409.1 + 78846 0.66 0.82531
Target:  5'- gAAGUGGGUUC-CGACAagGAGUGGcUGa -3'
miRNA:   3'- -UUCGCCCGGGcGCUGU--UUCACCaACa -5'
13309 5' -56.9 NC_003409.1 + 15874 0.66 0.807736
Target:  5'- uGGCGGucgGCCCuaaucgcuacGUGGCAAGGUGG-UGUa -3'
miRNA:   3'- uUCGCC---CGGG----------CGCUGUUUCACCaACA- -5'
13309 5' -56.9 NC_003409.1 + 112932 0.66 0.807736
Target:  5'- cAAGCGGGCgCGUuGCGcucGGGcGGUUGUg -3'
miRNA:   3'- -UUCGCCCGgGCGcUGU---UUCaCCAACA- -5'
13309 5' -56.9 NC_003409.1 + 57734 0.68 0.741536
Target:  5'- -cGCGGGCCCGCuugccuuugGGCugcggugcgagGAGGUGGUg-- -3'
miRNA:   3'- uuCGCCCGGGCG---------CUG-----------UUUCACCAaca -5'
13309 5' -56.9 NC_003409.1 + 8373 0.71 0.556756
Target:  5'- cAGCGGGCCCGUGAU-AAGU--UUGUa -3'
miRNA:   3'- uUCGCCCGGGCGCUGuUUCAccAACA- -5'
13309 5' -56.9 NC_003409.1 + 57883 1.04 0.003681
Target:  5'- cAAGCGGGCCCGCGACAAAGUGGUUGUu -3'
miRNA:   3'- -UUCGCCCGGGCGCUGUUUCACCAACA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.