miRNA: 3'- -UCUUGaGGCUGa--CaaGUGG--UCUUUGG- -5'
miRNA: 3'- -UCuUGAGgCUGAcaaGUGGUCUUUGG- -5'
miRNA: 3'- -UCUUG-AGGcUGacaa--GUGGUCUUUGG- -5'
miRNA: 3'- -UCUUGAGGc--UGAC-AAgUGG------UCUUUGG- -5'
miRNA: 3'- ucuUGAGGCugACaaGUGGUC-UUUGg -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13310 | 5' | -48.4 | NC_003409.1 | + | 118520 | 0.71 | 0.967079 |
|
Target: 5'- aGGAAC-CCGGCgcgGcgCACCccAGGAACCc -3' miRNA: 3'- -UCUUGaGGCUGa--CaaGUGG--UCUUUGG- -5' |
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| 13310 | 5' | -48.4 | NC_003409.1 | + | 50028 | 0.71 | 0.973221 |
|
Target: 5'- gAGuACUCaGACUcc-CACCAGAAGCCu -3' miRNA: 3'- -UCuUGAGgCUGAcaaGUGGUCUUUGG- -5' |
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| 13310 | 5' | -48.4 | NC_003409.1 | + | 31097 | 0.69 | 0.989733 |
|
Target: 5'- cAGAGCaUCCaACaacaggCACCGGAGACCa -3' miRNA: 3'- -UCUUG-AGGcUGacaa--GUGGUCUUUGG- -5' |
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| 13310 | 5' | -48.4 | NC_003409.1 | + | 119529 | 0.69 | 0.992217 |
|
Target: 5'- uAGAACUCCcaaGCUGaUUgGCCcagagcGGGAACCa -3' miRNA: 3'- -UCUUGAGGc--UGAC-AAgUGG------UCUUUGG- -5' |
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| 13310 | 5' | -48.4 | NC_003409.1 | + | 88258 | 0.66 | 0.999341 |
|
Target: 5'- --uGCUCCGugUGgaUACCAGuGAAUg -3' miRNA: 3'- ucuUGAGGCugACaaGUGGUC-UUUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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