miRNA: 3'- ucUUGAGGCUGa------CAAGUGG---UCUUUGG- -5'
miRNA: 3'- -UCuugAGGCUGa----CA----AGUGGUCUUUGG- -5'
miRNA: 3'- -UCUUGaGGCUGa--CaaGU-GGUCUUUGG- -5'
miRNA: 3'- ucuuGAGGCugACaagUGGUCUUUGG- -5'
miRNA: 3'- -UCUUGAGGCUGACAAGUGGUCUUUGG- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13310 | 5' | -48.4 | NC_003409.1 | + | 84993 | 0.72 | 0.95194 |
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Target: 5'- -uGACUCCGACcuggaugGUUCAUCgcaAGGAGCCc -3' miRNA: 3'- ucUUGAGGCUGa------CAAGUGG---UCUUUGG- -5' |
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| 13310 | 5' | -48.4 | NC_003409.1 | + | 64347 | 0.72 | 0.937921 |
|
Target: 5'- gAGua-UCCGGCcuaugGUuuuuUCACCAGAGGCCa -3' miRNA: 3'- -UCuugAGGCUGa----CA----AGUGGUCUUUGG- -5' |
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| 13310 | 5' | -48.4 | NC_003409.1 | + | 118565 | 0.73 | 0.915525 |
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Target: 5'- aGGAAC-CCGGCgcgGcgCAcCCAGGAACCc -3' miRNA: 3'- -UCUUGaGGCUGa--CaaGU-GGUCUUUGG- -5' |
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| 13310 | 5' | -48.4 | NC_003409.1 | + | 100536 | 0.74 | 0.881614 |
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Target: 5'- ---uCUCCGugUGa--ACCAGAGACCa -3' miRNA: 3'- ucuuGAGGCugACaagUGGUCUUUGG- -5' |
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| 13310 | 5' | -48.4 | NC_003409.1 | + | 59210 | 1.14 | 0.007346 |
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Target: 5'- uAGAACUCCGACUGUUCACCAGAAACCg -3' miRNA: 3'- -UCUUGAGGCUGACAAGUGGUCUUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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