miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
13311 5' -56.9 NC_003409.1 + 47535 0.66 0.862414
Target:  5'- uGGuUCCCaGGGGGUGUcuccGUCUGCUc -3'
miRNA:   3'- uCCcAGGG-CCCUCACAcu--UAGACGAc -5'
13311 5' -56.9 NC_003409.1 + 8967 0.66 0.853897
Target:  5'- gGGGGccuuagaggaagaUCCUGGGGGUGUGGgggaacuggacGUCUGa-- -3'
miRNA:   3'- -UCCC-------------AGGGCCCUCACACU-----------UAGACgac -5'
13311 5' -56.9 NC_003409.1 + 9253 0.66 0.838613
Target:  5'- cGGGUCUCGGGAGUucauacuuguugGUGAuggcggacUCUGUc- -3'
miRNA:   3'- uCCCAGGGCCCUCA------------CACUu-------AGACGac -5'
13311 5' -56.9 NC_003409.1 + 105210 0.68 0.72761
Target:  5'- uGGGGUCgCCGGGGGaGUGGgcgguggcucucuGUCaggccgUGCUGg -3'
miRNA:   3'- -UCCCAG-GGCCCUCaCACU-------------UAG------ACGAC- -5'
13311 5' -56.9 NC_003409.1 + 126637 0.69 0.667031
Target:  5'- gAGGaGUCgUGGGugAGUGUGGagguguaGUCUGCUGc -3'
miRNA:   3'- -UCC-CAGgGCCC--UCACACU-------UAGACGAC- -5'
13311 5' -56.9 NC_003409.1 + 63802 1.08 0.002162
Target:  5'- aAGGGUCCCGGGAGUGUGAAUCUGCUGa -3'
miRNA:   3'- -UCCCAGGGCCCUCACACUUAGACGAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.