Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13311 | 5' | -56.9 | NC_003409.1 | + | 63802 | 1.08 | 0.002162 |
Target: 5'- aAGGGUCCCGGGAGUGUGAAUCUGCUGa -3' miRNA: 3'- -UCCCAGGGCCCUCACACUUAGACGAC- -5' |
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13311 | 5' | -56.9 | NC_003409.1 | + | 126637 | 0.69 | 0.667031 |
Target: 5'- gAGGaGUCgUGGGugAGUGUGGagguguaGUCUGCUGc -3' miRNA: 3'- -UCC-CAGgGCCC--UCACACU-------UAGACGAC- -5' |
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13311 | 5' | -56.9 | NC_003409.1 | + | 105210 | 0.68 | 0.72761 |
Target: 5'- uGGGGUCgCCGGGGGaGUGGgcgguggcucucuGUCaggccgUGCUGg -3' miRNA: 3'- -UCCCAG-GGCCCUCaCACU-------------UAG------ACGAC- -5' |
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13311 | 5' | -56.9 | NC_003409.1 | + | 8967 | 0.66 | 0.853897 |
Target: 5'- gGGGGccuuagaggaagaUCCUGGGGGUGUGGgggaacuggacGUCUGa-- -3' miRNA: 3'- -UCCC-------------AGGGCCCUCACACU-----------UAGACgac -5' |
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13311 | 5' | -56.9 | NC_003409.1 | + | 9253 | 0.66 | 0.838613 |
Target: 5'- cGGGUCUCGGGAGUucauacuuguugGUGAuggcggacUCUGUc- -3' miRNA: 3'- uCCCAGGGCCCUCA------------CACUu-------AGACGac -5' |
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13311 | 5' | -56.9 | NC_003409.1 | + | 47535 | 0.66 | 0.862414 |
Target: 5'- uGGuUCCCaGGGGGUGUcuccGUCUGCUc -3' miRNA: 3'- uCCcAGGG-CCCUCACAcu--UAGACGAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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