Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13314 | 3' | -52 | NC_003409.1 | + | 64437 | 0.66 | 0.983512 |
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Target: 5'- cCCUCuauauuccGGGCUa--CUCAGAGGUgAGCa -3' miRNA: 3'- -GGAGc-------CUCGAguaGAGUCUCUAgUCG- -5' |
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| 13314 | 3' | -52 | NC_003409.1 | + | 42672 | 0.66 | 0.981487 |
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Target: 5'- gUCUgCGGA-CUCGUCgUCAGGGAggcgcUCGGUg -3' miRNA: 3'- -GGA-GCCUcGAGUAG-AGUCUCU-----AGUCG- -5' |
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| 13314 | 3' | -52 | NC_003409.1 | + | 64149 | 0.68 | 0.954195 |
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Target: 5'- aCCU-GGAGCaggCcUCUCAGAGAaCAGa -3' miRNA: 3'- -GGAgCCUCGa--GuAGAGUCUCUaGUCg -5' |
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| 13314 | 3' | -52 | NC_003409.1 | + | 35008 | 0.68 | 0.940966 |
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Target: 5'- gCCUU-GAGCUCGcugugacguUCUCAcgguguugguuGGGAUCAGCu -3' miRNA: 3'- -GGAGcCUCGAGU---------AGAGU-----------CUCUAGUCG- -5' |
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| 13314 | 3' | -52 | NC_003409.1 | + | 14360 | 0.7 | 0.887752 |
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Target: 5'- aCUCGGGguGCUCugccagCUCGGAGcgCAGg -3' miRNA: 3'- gGAGCCU--CGAGua----GAGUCUCuaGUCg -5' |
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| 13314 | 3' | -52 | NC_003409.1 | + | 66409 | 0.72 | 0.806539 |
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Target: 5'- gUCUCaaGAGCUgAUCUCuGAGAUgAGCu -3' miRNA: 3'- -GGAGc-CUCGAgUAGAGuCUCUAgUCG- -5' |
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| 13314 | 3' | -52 | NC_003409.1 | + | 82399 | 0.73 | 0.768917 |
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Target: 5'- aCCaCGGAugcGCUCGUCUgcCGGAGGcUCGGCg -3' miRNA: 3'- -GGaGCCU---CGAGUAGA--GUCUCU-AGUCG- -5' |
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| 13314 | 3' | -52 | NC_003409.1 | + | 115079 | 0.77 | 0.562405 |
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Target: 5'- uUUUGGAGCUCAUCUCGGuGA-CGGg -3' miRNA: 3'- gGAGCCUCGAGUAGAGUCuCUaGUCg -5' |
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| 13314 | 3' | -52 | NC_003409.1 | + | 66581 | 1.14 | 0.003199 |
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Target: 5'- uCCUCGGAGCUCAUCUCAGAGAUCAGCu -3' miRNA: 3'- -GGAGCCUCGAGUAGAGUCUCUAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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