Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13315 | 3' | -56.2 | NC_003409.1 | + | 132321 | 0.66 | 0.903124 |
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Target: 5'- aCGUAgUGCUaACUGGcGCCGUggcguGGAGUCCa -3' miRNA: 3'- -GCAU-ACGG-UGAUC-UGGCA-----CCUCGGGc -5' |
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| 13315 | 3' | -56.2 | NC_003409.1 | + | 130572 | 0.66 | 0.901224 |
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Target: 5'- gCGUGUcgcccGUCACUGGAuguaaguccggccaCCGUGGAguuGCCUGu -3' miRNA: 3'- -GCAUA-----CGGUGAUCU--------------GGCACCU---CGGGC- -5' |
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| 13315 | 3' | -56.2 | NC_003409.1 | + | 133609 | 0.66 | 0.890061 |
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Target: 5'- uGUAUGCCACcgaACCGgcccugGGAcCCCGu -3' miRNA: 3'- gCAUACGGUGaucUGGCa-----CCUcGGGC- -5' |
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| 13315 | 3' | -56.2 | NC_003409.1 | + | 61460 | 0.66 | 0.868774 |
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Target: 5'- --aGUGCCGuaGGAgCGUGGAGCUUa -3' miRNA: 3'- gcaUACGGUgaUCUgGCACCUCGGGc -5' |
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| 13315 | 3' | -56.2 | NC_003409.1 | + | 27950 | 0.67 | 0.829121 |
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Target: 5'- aGUGcacGCCGCUaggugGGACCGUGGgcgAGCCg- -3' miRNA: 3'- gCAUa--CGGUGA-----UCUGGCACC---UCGGgc -5' |
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| 13315 | 3' | -56.2 | NC_003409.1 | + | 34471 | 0.68 | 0.811953 |
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Target: 5'- aGUGguUGCCAUccuuUGGGCCGUGGuucaucAGCCUc -3' miRNA: 3'- gCAU--ACGGUG----AUCUGGCACC------UCGGGc -5' |
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| 13315 | 3' | -56.2 | NC_003409.1 | + | 115023 | 0.68 | 0.794131 |
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Target: 5'- ----gGCCACUGGACgGUGGuuuCCCu -3' miRNA: 3'- gcauaCGGUGAUCUGgCACCuc-GGGc -5' |
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| 13315 | 3' | -56.2 | NC_003409.1 | + | 109846 | 0.69 | 0.737435 |
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Target: 5'- ----cGCCGCggcgggAGGCgGUGGcGCCCGg -3' miRNA: 3'- gcauaCGGUGa-----UCUGgCACCuCGGGC- -5' |
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| 13315 | 3' | -56.2 | NC_003409.1 | + | 9627 | 0.71 | 0.61607 |
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Target: 5'- cCGUGUGuCCGCUGGcACUGUGGAaaaccuuCCCGc -3' miRNA: 3'- -GCAUAC-GGUGAUC-UGGCACCUc------GGGC- -5' |
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| 13315 | 3' | -56.2 | NC_003409.1 | + | 66847 | 1.1 | 0.002331 |
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Target: 5'- gCGUAUGCCACUAGACCGUGGAGCCCGg -3' miRNA: 3'- -GCAUACGGUGAUCUGGCACCUCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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