Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13318 | 5' | -66.2 | NC_003409.1 | + | 109478 | 0.69 | 0.300522 |
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Target: 5'- cGGCUCcCGUGG-GCCCaagaCgGCGCGCc -3' miRNA: 3'- cCCGGGaGCACCgCGGGg---GaCGCGCG- -5' |
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| 13318 | 5' | -66.2 | NC_003409.1 | + | 31949 | 0.69 | 0.287704 |
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Target: 5'- cGGCCacugugccgCGUcGGCGCCCCa-GgGCGCa -3' miRNA: 3'- cCCGGga-------GCA-CCGCGGGGgaCgCGCG- -5' |
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| 13318 | 5' | -66.2 | NC_003409.1 | + | 78774 | 0.69 | 0.283943 |
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Target: 5'- cGGGUCUUCGgugGGUggcuuggguaaaguuGCCUCCggcugGCGCGUa -3' miRNA: 3'- -CCCGGGAGCa--CCG---------------CGGGGGa----CGCGCG- -5' |
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| 13318 | 5' | -66.2 | NC_003409.1 | + | 87186 | 0.7 | 0.263366 |
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Target: 5'- aGGGCgUggcCGacGCGCCaCCCUGCGUGUg -3' miRNA: 3'- -CCCGgGa--GCacCGCGG-GGGACGCGCG- -5' |
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| 13318 | 5' | -66.2 | NC_003409.1 | + | 98723 | 0.7 | 0.257548 |
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Target: 5'- cGGCCUguuggcgucCGUGGUGCCaaCCCU-CGCGCu -3' miRNA: 3'- cCCGGGa--------GCACCGCGG--GGGAcGCGCG- -5' |
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| 13318 | 5' | -66.2 | NC_003409.1 | + | 41857 | 0.71 | 0.230028 |
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Target: 5'- cGGCCaCUCGUGcaGCGgacugcaCCCCUGCGCa- -3' miRNA: 3'- cCCGG-GAGCAC--CGCg------GGGGACGCGcg -5' |
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| 13318 | 5' | -66.2 | NC_003409.1 | + | 67977 | 1.11 | 0.000254 |
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Target: 5'- aGGGCCCUCGUGGCGCCCCCUGCGCGCc -3' miRNA: 3'- -CCCGGGAGCACCGCGGGGGACGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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