Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13319 | 3' | -51.8 | NC_003409.1 | + | 55722 | 0.66 | 0.974301 |
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Target: 5'- cAUGCcgucaGGGCCCACGGUGcacuGAa-- -3' miRNA: 3'- aUACGa----CCUGGGUGCCACuau-CUaua -5' |
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| 13319 | 3' | -51.8 | NC_003409.1 | + | 15757 | 0.66 | 0.968313 |
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Target: 5'- gGUGCUGGcuggcuGCCUuacCGG-GGUGGAUAUa -3' miRNA: 3'- aUACGACC------UGGGu--GCCaCUAUCUAUA- -5' |
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| 13319 | 3' | -51.8 | NC_003409.1 | + | 81550 | 0.67 | 0.95757 |
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Target: 5'- --cGCUGuGGCCCGgGGagUGAUGGAg-- -3' miRNA: 3'- auaCGAC-CUGGGUgCC--ACUAUCUaua -5' |
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| 13319 | 3' | -51.8 | NC_003409.1 | + | 98622 | 0.67 | 0.95757 |
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Target: 5'- --cGCUcGACCCcCGGUGuUGGAUGg -3' miRNA: 3'- auaCGAcCUGGGuGCCACuAUCUAUa -5' |
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| 13319 | 3' | -51.8 | NC_003409.1 | + | 66667 | 0.67 | 0.953495 |
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Target: 5'- aGUGUUGGaagaGCCgCugGGUGggGGAUGg -3' miRNA: 3'- aUACGACC----UGG-GugCCACuaUCUAUa -5' |
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| 13319 | 3' | -51.8 | NC_003409.1 | + | 47921 | 0.68 | 0.928656 |
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Target: 5'- -cUGCcGGAcacugaaCCCugGGUGGUAGAg-- -3' miRNA: 3'- auACGaCCU-------GGGugCCACUAUCUaua -5' |
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| 13319 | 3' | -51.8 | NC_003409.1 | + | 131099 | 0.69 | 0.89129 |
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Target: 5'- -cUGCUGGugGCCCugGGUGuaGUGGGc-- -3' miRNA: 3'- auACGACC--UGGGugCCAC--UAUCUaua -5' |
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| 13319 | 3' | -51.8 | NC_003409.1 | + | 107697 | 0.71 | 0.817741 |
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Target: 5'- --cGCUGGACCCACGGccgccgGAaGGGg-- -3' miRNA: 3'- auaCGACCUGGGUGCCa-----CUaUCUaua -5' |
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| 13319 | 3' | -51.8 | NC_003409.1 | + | 85879 | 0.75 | 0.57879 |
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Target: 5'- --aGC-GGAUCCGCGGUGAUAGGa-- -3' miRNA: 3'- auaCGaCCUGGGUGCCACUAUCUaua -5' |
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| 13319 | 3' | -51.8 | NC_003409.1 | + | 68496 | 1.05 | 0.009497 |
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Target: 5'- cUAUGCUGGACCCACGGUGAUAGAUAUg -3' miRNA: 3'- -AUACGACCUGGGUGCCACUAUCUAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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