Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13319 | 5' | -57 | NC_003409.1 | + | 76965 | 0.66 | 0.868864 |
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Target: 5'- uCGACGUCUgcaUGUcagaCGGGGGCACcGCUAa -3' miRNA: 3'- -GCUGCAGAa--GCG----GCUCCUGUG-CGGUa -5' |
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| 13319 | 5' | -57 | NC_003409.1 | + | 80196 | 0.66 | 0.837679 |
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Target: 5'- gCGACGaa--CG-CGGGGACGCGCCGc -3' miRNA: 3'- -GCUGCagaaGCgGCUCCUGUGCGGUa -5' |
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| 13319 | 5' | -57 | NC_003409.1 | + | 107345 | 0.68 | 0.766914 |
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Target: 5'- -aGCGUCUccaaCGCCGAuGGAauacaGCGCCAg -3' miRNA: 3'- gcUGCAGAa---GCGGCU-CCUg----UGCGGUa -5' |
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| 13319 | 5' | -57 | NC_003409.1 | + | 58320 | 0.68 | 0.738172 |
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Target: 5'- gGGCGUUaauuuccucuuUUUG-CGAGGACACGCCu- -3' miRNA: 3'- gCUGCAG-----------AAGCgGCUCCUGUGCGGua -5' |
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| 13319 | 5' | -57 | NC_003409.1 | + | 58030 | 0.7 | 0.647964 |
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Target: 5'- uGACGU--UCGCCGcgaaauccAGcGACACGCCAa -3' miRNA: 3'- gCUGCAgaAGCGGC--------UC-CUGUGCGGUa -5' |
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| 13319 | 5' | -57 | NC_003409.1 | + | 82615 | 0.74 | 0.406582 |
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Target: 5'- gCGugGUCUgCGCCGAGGACGCuuuUCAg -3' miRNA: 3'- -GCugCAGAaGCGGCUCCUGUGc--GGUa -5' |
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| 13319 | 5' | -57 | NC_003409.1 | + | 81176 | 0.75 | 0.357941 |
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Target: 5'- gCGACGUCUUCGCCGcgccacucguccgguGGACAgGCUg- -3' miRNA: 3'- -GCUGCAGAAGCGGCu--------------CCUGUgCGGua -5' |
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| 13319 | 5' | -57 | NC_003409.1 | + | 68458 | 1.06 | 0.003635 |
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Target: 5'- aCGACGUCUUCGCCGAGGACACGCCAUc -3' miRNA: 3'- -GCUGCAGAAGCGGCUCCUGUGCGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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