Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1332 | 5' | -55.7 | NC_001335.1 | + | 24794 | 0.66 | 0.673157 |
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Target: 5'- gACGaugUCCAGCUCcucaGGCGUCAGCGc-- -3' miRNA: 3'- aUGCa--GGGUCGAG----CUGUAGUCGCuca -5' |
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| 1332 | 5' | -55.7 | NC_001335.1 | + | 17572 | 0.66 | 0.662082 |
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Target: 5'- -uCGUCCagGGCcugaucaaCGGCAUCGGCGGGa -3' miRNA: 3'- auGCAGGg-UCGa-------GCUGUAGUCGCUCa -5' |
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| 1332 | 5' | -55.7 | NC_001335.1 | + | 42368 | 0.66 | 0.648755 |
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Target: 5'- gGCGUCCaaGGCUCGcugcaucugguuCAUCAGcCGGGUg -3' miRNA: 3'- aUGCAGGg-UCGAGCu-----------GUAGUC-GCUCA- -5' |
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| 1332 | 5' | -55.7 | NC_001335.1 | + | 34175 | 0.67 | 0.606489 |
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Target: 5'- -uCGUCCCAGUgcaucaUCGACAUCcGCGc-- -3' miRNA: 3'- auGCAGGGUCG------AGCUGUAGuCGCuca -5' |
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| 1332 | 5' | -55.7 | NC_001335.1 | + | 14433 | 0.67 | 0.562355 |
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Target: 5'- cACGcUCCCugAGUUCGGCuucgaaggCGGCGAGUc -3' miRNA: 3'- aUGC-AGGG--UCGAGCUGua------GUCGCUCA- -5' |
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| 1332 | 5' | -55.7 | NC_001335.1 | + | 45532 | 0.68 | 0.519133 |
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Target: 5'- cACGUCCUcguaCUCGACGgUCAGCGAc- -3' miRNA: 3'- aUGCAGGGuc--GAGCUGU-AGUCGCUca -5' |
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| 1332 | 5' | -55.7 | NC_001335.1 | + | 21747 | 0.68 | 0.508526 |
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Target: 5'- aGCGUCaaGGCa-GACAUCAGCGAc- -3' miRNA: 3'- aUGCAGggUCGagCUGUAGUCGCUca -5' |
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| 1332 | 5' | -55.7 | NC_001335.1 | + | 22199 | 0.69 | 0.46708 |
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Target: 5'- aGCGUcugcugaacaaCCCGGC-CGAgguCAUCGGCGAGa -3' miRNA: 3'- aUGCA-----------GGGUCGaGCU---GUAGUCGCUCa -5' |
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| 1332 | 5' | -55.7 | NC_001335.1 | + | 5459 | 0.81 | 0.084318 |
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Target: 5'- -uUGUCCC-GCUCGAUGUCGGCGAGg -3' miRNA: 3'- auGCAGGGuCGAGCUGUAGUCGCUCa -5' |
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| 1332 | 5' | -55.7 | NC_001335.1 | + | 1865 | 1.05 | 0.001395 |
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Target: 5'- aUACGUCCCAGCUCGACAUCAGCGAGUc -3' miRNA: 3'- -AUGCAGGGUCGAGCUGUAGUCGCUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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