Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13320 | 3' | -53.3 | NC_003409.1 | + | 19159 | 0.66 | 0.957726 |
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Target: 5'- -aGGACGAggagggGCCCgc-GGGGGCa--- -3' miRNA: 3'- caCCUGCUa-----CGGGacuUCCCUGaaag -5' |
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| 13320 | 3' | -53.3 | NC_003409.1 | + | 27382 | 0.66 | 0.945087 |
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Target: 5'- cGUGGACGGggGCCauCUGGAGGuGCcaagUUCg -3' miRNA: 3'- -CACCUGCUa-CGG--GACUUCCcUGa---AAG- -5' |
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| 13320 | 3' | -53.3 | NC_003409.1 | + | 7060 | 0.67 | 0.93743 |
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Target: 5'- -cGGGCaGUGCUCUGGAGGGuuuaggggagguauuGCgUUUCg -3' miRNA: 3'- caCCUGcUACGGGACUUCCC---------------UG-AAAG- -5' |
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| 13320 | 3' | -53.3 | NC_003409.1 | + | 110952 | 0.69 | 0.848158 |
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Target: 5'- -cGGGCGAUGUgCUGggGGuGAUa--- -3' miRNA: 3'- caCCUGCUACGgGACuuCC-CUGaaag -5' |
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| 13320 | 3' | -53.3 | NC_003409.1 | + | 111714 | 0.7 | 0.785858 |
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Target: 5'- --cGGCGAUGUCCUGAuuuuccAGGGACa--- -3' miRNA: 3'- cacCUGCUACGGGACU------UCCCUGaaag -5' |
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| 13320 | 3' | -53.3 | NC_003409.1 | + | 77000 | 1.11 | 0.003262 |
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Target: 5'- aGUGGACGAUGCCCUGAAGGGACUUUCg -3' miRNA: 3'- -CACCUGCUACGGGACUUCCCUGAAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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