Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13320 | 5' | -59 | NC_003409.1 | + | 88685 | 0.66 | 0.711346 |
Target: 5'- gCUGCcgG-CAGGCaGGGGCGCggggGCUGc -3' miRNA: 3'- -GACGuaCaGUCUGcCCCCGUGg---CGAU- -5' |
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13320 | 5' | -59 | NC_003409.1 | + | 107485 | 0.67 | 0.650599 |
Target: 5'- -cGC---UUGGACGGGGGCGuCCGCg- -3' miRNA: 3'- gaCGuacAGUCUGCCCCCGU-GGCGau -5' |
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13320 | 5' | -59 | NC_003409.1 | + | 111153 | 0.68 | 0.630141 |
Target: 5'- uUGCGUGUCGGGa--GGGCGCCGg-- -3' miRNA: 3'- gACGUACAGUCUgccCCCGUGGCgau -5' |
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13320 | 5' | -59 | NC_003409.1 | + | 123748 | 0.68 | 0.609689 |
Target: 5'- gUGCugGUGUguGugGGGGGCAgUGUUc -3' miRNA: 3'- gACG--UACAguCugCCCCCGUgGCGAu -5' |
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13320 | 5' | -59 | NC_003409.1 | + | 119793 | 0.71 | 0.443631 |
Target: 5'- -cGCGUGcCAGGCGccggcguGGGCGCCGCg- -3' miRNA: 3'- gaCGUACaGUCUGCc------CCCGUGGCGau -5' |
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13320 | 5' | -59 | NC_003409.1 | + | 76959 | 1.06 | 0.00175 |
Target: 5'- uCUGCAUGUCAGACGGGGGCACCGCUAa -3' miRNA: 3'- -GACGUACAGUCUGCCCCCGUGGCGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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