Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13325 | 5' | -57.3 | NC_003409.1 | + | 109062 | 0.67 | 0.795714 |
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Target: 5'- -aUCGUgagAGCCGCUCCaaguugucgaaaGGUCCCGc-- -3' miRNA: 3'- cgGGCAa--UCGGUGAGG------------CCAGGGCuuu -5' |
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| 13325 | 5' | -57.3 | NC_003409.1 | + | 133613 | 0.67 | 0.777367 |
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Target: 5'- gGCCUGUauGCCACcgaaCCGGcCCUGGGAc -3' miRNA: 3'- -CGGGCAauCGGUGa---GGCCaGGGCUUU- -5' |
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| 13325 | 5' | -57.3 | NC_003409.1 | + | 84947 | 0.69 | 0.658924 |
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Target: 5'- cGCCUGc-GGCUGCagaacccCCGGUCCCGGAGg -3' miRNA: 3'- -CGGGCaaUCGGUGa------GGCCAGGGCUUU- -5' |
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| 13325 | 5' | -57.3 | NC_003409.1 | + | 94888 | 0.71 | 0.55705 |
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Target: 5'- gGCCuCGaucGGCCugcugACUCCGGUCCUGAu- -3' miRNA: 3'- -CGG-GCaa-UCGG-----UGAGGCCAGGGCUuu -5' |
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| 13325 | 5' | -57.3 | NC_003409.1 | + | 112131 | 0.72 | 0.497947 |
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Target: 5'- cGCCCGggGGCCaggACUCacugggGGUCUCGGGAa -3' miRNA: 3'- -CGGGCaaUCGG---UGAGg-----CCAGGGCUUU- -5' |
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| 13325 | 5' | -57.3 | NC_003409.1 | + | 86734 | 1.08 | 0.002105 |
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Target: 5'- cGCCCGUUAGCCACUCCGGUCCCGAAAa -3' miRNA: 3'- -CGGGCAAUCGGUGAGGCCAGGGCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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