Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1333 | 3' | -55.1 | NC_001335.1 | + | 32319 | 0.66 | 0.713282 |
Target: 5'- gAGUUGGUGGaac-GAGGucaGCCaGGGGGCa -3' miRNA: 3'- -UCAACCGUCgucuUUCC---UGG-CCCUCG- -5' |
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1333 | 3' | -55.1 | NC_001335.1 | + | 29751 | 0.66 | 0.691431 |
Target: 5'- uGGUUGGCcgauCAG-GAGGuCUGGGAGg -3' miRNA: 3'- -UCAACCGuc--GUCuUUCCuGGCCCUCg -5' |
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1333 | 3' | -55.1 | NC_001335.1 | + | 17494 | 0.66 | 0.680412 |
Target: 5'- ---aGGCAGCGGAAcu-GCCGGGGa- -3' miRNA: 3'- ucaaCCGUCGUCUUuccUGGCCCUcg -5' |
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1333 | 3' | -55.1 | NC_001335.1 | + | 9871 | 0.66 | 0.669345 |
Target: 5'- --gUGGCuucGGCGGugccguGGGGCUcgGGGAGCc -3' miRNA: 3'- ucaACCG---UCGUCuu----UCCUGG--CCCUCG- -5' |
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1333 | 3' | -55.1 | NC_001335.1 | + | 16059 | 0.66 | 0.669345 |
Target: 5'- ---cGGCGGCAGccGGGGGCUcGGAGa -3' miRNA: 3'- ucaaCCGUCGUCu-UUCCUGGcCCUCg -5' |
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1333 | 3' | -55.1 | NC_001335.1 | + | 35784 | 0.66 | 0.658243 |
Target: 5'- cGggGGCuauGCAGAGGcGAUCaGGAGCc -3' miRNA: 3'- uCaaCCGu--CGUCUUUcCUGGcCCUCG- -5' |
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1333 | 3' | -55.1 | NC_001335.1 | + | 39294 | 0.68 | 0.569396 |
Target: 5'- ---gGGCGGCGaAGAGGucuccgggguACCGGGuGCa -3' miRNA: 3'- ucaaCCGUCGUcUUUCC----------UGGCCCuCG- -5' |
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1333 | 3' | -55.1 | NC_001335.1 | + | 50200 | 0.69 | 0.525933 |
Target: 5'- cGUccgGGCAGgAGAAGccuuGACCGGaGGGCa -3' miRNA: 3'- uCAa--CCGUCgUCUUUc---CUGGCC-CUCG- -5' |
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1333 | 3' | -55.1 | NC_001335.1 | + | 15743 | 0.69 | 0.493128 |
Target: 5'- ---aGGCGGCucgagagAGAAGGGAaaGGGAGg -3' miRNA: 3'- ucaaCCGUCG-------UCUUUCCUggCCCUCg -5' |
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1333 | 3' | -55.1 | NC_001335.1 | + | 15528 | 0.7 | 0.431549 |
Target: 5'- uGGUgGGCAGCAGuaccggggcuGGACCGaGGAccGCu -3' miRNA: 3'- -UCAaCCGUCGUCuuu-------CCUGGC-CCU--CG- -5' |
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1333 | 3' | -55.1 | NC_001335.1 | + | 2351 | 0.73 | 0.302841 |
Target: 5'- ---cGGCGaCAGGaacuaccaGAGGACUGGGAGCu -3' miRNA: 3'- ucaaCCGUcGUCU--------UUCCUGGCCCUCG- -5' |
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1333 | 3' | -55.1 | NC_001335.1 | + | 1254 | 0.75 | 0.227792 |
Target: 5'- --gUGGCGGCGGAAGuGGAgCCGGcGGCg -3' miRNA: 3'- ucaACCGUCGUCUUU-CCU-GGCCcUCG- -5' |
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1333 | 3' | -55.1 | NC_001335.1 | + | 7456 | 0.75 | 0.221812 |
Target: 5'- uGUgGGCGuuGCAGAucgacAGGGugCGGGAGCc -3' miRNA: 3'- uCAaCCGU--CGUCU-----UUCCugGCCCUCG- -5' |
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1333 | 3' | -55.1 | NC_001335.1 | + | 2469 | 1.11 | 0.000661 |
Target: 5'- cAGUUGGCAGCAGAAAGGACCGGGAGCc -3' miRNA: 3'- -UCAACCGUCGUCUUUCCUGGCCCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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