Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13332 | 5' | -57 | NC_003409.1 | + | 3693 | 0.66 | 0.84537 |
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Target: 5'- aGACUguACGGCGgcaaacUGGUGC-CCG-UGCCc -3' miRNA: 3'- -UUGG--UGCCGCa-----ACCAUGaGGCgACGG- -5' |
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| 13332 | 5' | -57 | NC_003409.1 | + | 56614 | 0.66 | 0.837199 |
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Target: 5'- cGCCAUuaGGCc--GGUgaACUCCGCcGCCa -3' miRNA: 3'- uUGGUG--CCGcaaCCA--UGAGGCGaCGG- -5' |
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| 13332 | 5' | -57 | NC_003409.1 | + | 9618 | 0.67 | 0.793672 |
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Target: 5'- aGACCACGGcCGU--GUG-UCCGCUGgCa -3' miRNA: 3'- -UUGGUGCC-GCAacCAUgAGGCGACgG- -5' |
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| 13332 | 5' | -57 | NC_003409.1 | + | 109846 | 0.67 | 0.784491 |
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Target: 5'- cGCCGCGGCGggaggcggUGGcGC-CCGgcgcaacaCUGCCa -3' miRNA: 3'- uUGGUGCCGCa-------ACCaUGaGGC--------GACGG- -5' |
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| 13332 | 5' | -57 | NC_003409.1 | + | 79679 | 0.68 | 0.716861 |
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Target: 5'- aGACCGCGGgGUUGuucaagCCGCUGUUg -3' miRNA: 3'- -UUGGUGCCgCAACcauga-GGCGACGG- -5' |
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| 13332 | 5' | -57 | NC_003409.1 | + | 107805 | 0.74 | 0.411474 |
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Target: 5'- aGGCCACGGUGggcacgccccUGGaGCUCC-CUGCCa -3' miRNA: 3'- -UUGGUGCCGCa---------ACCaUGAGGcGACGG- -5' |
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| 13332 | 5' | -57 | NC_003409.1 | + | 107669 | 1.1 | 0.001619 |
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Target: 5'- gAACCACGGCGUUGGUACUCCGCUGCCa -3' miRNA: 3'- -UUGGUGCCGCAACCAUGAGGCGACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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