Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1334 | 3' | -53 | NC_001335.1 | + | 17035 | 0.66 | 0.813374 |
Target: 5'- cGGUGAGCauCGuGCCGAGCgUGCUcgccaGCGu -3' miRNA: 3'- -CCAUUUGc-GC-CGGCUCG-ACGAaa---CGU- -5' |
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1334 | 3' | -53 | NC_001335.1 | + | 19595 | 0.66 | 0.813374 |
Target: 5'- aGGUAAugGCucaGUgaGAGCUGCaaggUGCAg -3' miRNA: 3'- -CCAUUugCGc--CGg-CUCGACGaa--ACGU- -5' |
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1334 | 3' | -53 | NC_001335.1 | + | 51841 | 0.67 | 0.762956 |
Target: 5'- aGUGAgcagccuauGCGCGGCaCGGGCUGCc----- -3' miRNA: 3'- cCAUU---------UGCGCCG-GCUCGACGaaacgu -5' |
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1334 | 3' | -53 | NC_001335.1 | + | 48117 | 0.67 | 0.749206 |
Target: 5'- aGGUAGACGCGGUCagcucccaggaacgGAGcCUGUguguugGCGu -3' miRNA: 3'- -CCAUUUGCGCCGG--------------CUC-GACGaaa---CGU- -5' |
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1334 | 3' | -53 | NC_001335.1 | + | 13704 | 0.68 | 0.705685 |
Target: 5'- cGGUGAugGUGGCCGGGaaguagcccucgucgGUgaUGCGg -3' miRNA: 3'- -CCAUUugCGCCGGCUCga-------------CGaaACGU- -5' |
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1334 | 3' | -53 | NC_001335.1 | + | 18611 | 0.68 | 0.686775 |
Target: 5'- --gGAGCGCGGCCc--CUGCUUcGCGu -3' miRNA: 3'- ccaUUUGCGCCGGcucGACGAAaCGU- -5' |
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1334 | 3' | -53 | NC_001335.1 | + | 33470 | 0.69 | 0.653051 |
Target: 5'- ---cAGCGacauGGCCGAGCUGCggagguugGCAg -3' miRNA: 3'- ccauUUGCg---CCGGCUCGACGaaa-----CGU- -5' |
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1334 | 3' | -53 | NC_001335.1 | + | 42285 | 0.69 | 0.607849 |
Target: 5'- cGUGacGACGCGGCagagGAGCUGCac-GCGc -3' miRNA: 3'- cCAU--UUGCGCCGg---CUCGACGaaaCGU- -5' |
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1334 | 3' | -53 | NC_001335.1 | + | 24457 | 0.69 | 0.607849 |
Target: 5'- uGGUGAACGCGgacuucgacaGCCG-GUUGCccUGCGu -3' miRNA: 3'- -CCAUUUGCGC----------CGGCuCGACGaaACGU- -5' |
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1334 | 3' | -53 | NC_001335.1 | + | 33846 | 0.7 | 0.562994 |
Target: 5'- aGGUuGAUGUGGCCGAGgUUGCacgGCu -3' miRNA: 3'- -CCAuUUGCGCCGGCUC-GACGaaaCGu -5' |
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1334 | 3' | -53 | NC_001335.1 | + | 7133 | 0.7 | 0.551905 |
Target: 5'- cGGUGAuccACGCGGCUGAacGCgGCU-UGCc -3' miRNA: 3'- -CCAUU---UGCGCCGGCU--CGaCGAaACGu -5' |
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1334 | 3' | -53 | NC_001335.1 | + | 17782 | 0.71 | 0.497616 |
Target: 5'- aGGaucuCGCGGCUGAGCUGUccaggGCGa -3' miRNA: 3'- -CCauuuGCGCCGGCUCGACGaaa--CGU- -5' |
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1334 | 3' | -53 | NC_001335.1 | + | 16471 | 0.72 | 0.445851 |
Target: 5'- gGGUGAuCGCggucagcaucgGGCCGAGCUGCUg---- -3' miRNA: 3'- -CCAUUuGCG-----------CCGGCUCGACGAaacgu -5' |
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1334 | 3' | -53 | NC_001335.1 | + | 32915 | 0.73 | 0.416273 |
Target: 5'- cGGU-GACGaCGGCCucGGCUGCUgUGCGc -3' miRNA: 3'- -CCAuUUGC-GCCGGc-UCGACGAaACGU- -5' |
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1334 | 3' | -53 | NC_001335.1 | + | 4185 | 1.11 | 0.000987 |
Target: 5'- cGGUAAACGCGGCCGAGCUGCUUUGCAu -3' miRNA: 3'- -CCAUUUGCGCCGGCUCGACGAAACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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