Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13342 | 3' | -48.1 | NC_003409.1 | + | 20201 | 0.66 | 0.998585 |
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Target: 5'- cGCgucAGCUGcAGCCGUAGUgGCUCUAu- -3' miRNA: 3'- uUGa--UCGAU-UUGGCGUCA-UGAGAUcc -5' |
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| 13342 | 3' | -48.1 | NC_003409.1 | + | 123516 | 0.67 | 0.997513 |
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Target: 5'- cGACgacGCUGGGCCGCgagAGUACgCUAGc -3' miRNA: 3'- -UUGau-CGAUUUGGCG---UCAUGaGAUCc -5' |
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| 13342 | 3' | -48.1 | NC_003409.1 | + | 121297 | 0.67 | 0.995835 |
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Target: 5'- ---cAGCUAGGCCuCAGUAUUCUAu- -3' miRNA: 3'- uugaUCGAUUUGGcGUCAUGAGAUcc -5' |
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| 13342 | 3' | -48.1 | NC_003409.1 | + | 86165 | 0.72 | 0.936143 |
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Target: 5'- gAAC-AGCU--GCUGCGGaACUCUGGGg -3' miRNA: 3'- -UUGaUCGAuuUGGCGUCaUGAGAUCC- -5' |
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| 13342 | 3' | -48.1 | NC_003409.1 | + | 102138 | 0.72 | 0.936143 |
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Target: 5'- aGGCU-GCUAAGCUGCGGU-CUCUcGGc -3' miRNA: 3'- -UUGAuCGAUUUGGCGUCAuGAGAuCC- -5' |
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| 13342 | 3' | -48.1 | NC_003409.1 | + | 121497 | 1.1 | 0.011356 |
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Target: 5'- uAACUAGCUAAACCGCAGUACUCUAGGg -3' miRNA: 3'- -UUGAUCGAUUUGGCGUCAUGAGAUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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