Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13342 | 5' | -48.8 | NC_003409.1 | + | 68742 | 0.66 | 0.995929 |
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Target: 5'- gGCGUgAGAcuguggACUGGGGCCU-GUUGGa -3' miRNA: 3'- aUGUA-UCUua----UGACUCCGGAuCGACU- -5' |
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| 13342 | 5' | -48.8 | NC_003409.1 | + | 13274 | 0.66 | 0.995207 |
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Target: 5'- cGCuUGGAugAUACUGGGGUacaAGCUGGc -3' miRNA: 3'- aUGuAUCU--UAUGACUCCGga-UCGACU- -5' |
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| 13342 | 5' | -48.8 | NC_003409.1 | + | 117609 | 0.66 | 0.995207 |
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Target: 5'- aUGCGgAGAGgaaUGGuGGCCUGGUUGAc -3' miRNA: 3'- -AUGUaUCUUaugACU-CCGGAUCGACU- -5' |
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| 13342 | 5' | -48.8 | NC_003409.1 | + | 121330 | 0.67 | 0.994385 |
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Target: 5'- aUGCccUAGAGUACUGcGGUUUAGCUa- -3' miRNA: 3'- -AUGu-AUCUUAUGACuCCGGAUCGAcu -5' |
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| 13342 | 5' | -48.8 | NC_003409.1 | + | 83642 | 0.68 | 0.981687 |
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Target: 5'- uUACAUGGAAUACgggagacacucugcaUGGGGUUUGGCa-- -3' miRNA: 3'- -AUGUAUCUUAUG---------------ACUCCGGAUCGacu -5' |
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| 13342 | 5' | -48.8 | NC_003409.1 | + | 121459 | 1.08 | 0.011425 |
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Target: 5'- uUACAUAGAAUACUGAGGCCUAGCUGAu -3' miRNA: 3'- -AUGUAUCUUAUGACUCCGGAUCGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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