miRNA: 3'- -GUCGUCCu--CGGUGuUGUCGUCcUCGG- -5'
miRNA: 3'- guCGUCCUcGGUGU-UGUCguCCU-CGg -5'
miRNA: 3'- -GUCGUCCu------CGGUGUUgUCGUCCUCGG- -5'
Results 101 - 103 of 103 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13345 | 3' | -57 | NC_003409.1 | + | 131523 | 0.68 | 0.6967 |
|
Target: 5'- cCAGUGGGuguGCCAC-GCAGCGGuAGUCc -3' miRNA: 3'- -GUCGUCCu--CGGUGuUGUCGUCcUCGG- -5' |
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| 13345 | 3' | -57 | NC_003409.1 | + | 132962 | 0.66 | 0.803445 |
|
Target: 5'- -cGCGGGAuCCGCAgGCAGguGGGuGCg -3' miRNA: 3'- guCGUCCUcGGUGU-UGUCguCCU-CGg -5' |
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| 13345 | 3' | -57 | NC_003409.1 | + | 133539 | 0.78 | 0.229177 |
|
Target: 5'- aCAGCAGGccgagauGCUGCAGgAGCGGGAGUCc -3' miRNA: 3'- -GUCGUCCu------CGGUGUUgUCGUCCUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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