miRNA: 3'- gucGUCCuCGGU-GUUGUCGuCC-UCGG- -5'
miRNA: 3'- gUCGUCc-UCGg--UGUUG-UCGUCCU-CGG- -5'
miRNA: 3'- -GUCG-----UCCUCGGuGUUGUCG-----UCCUCGG- -5'
Results 101 - 103 of 103 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13345 | 3' | -57 | NC_003409.1 | + | 130947 | 0.69 | 0.665887 |
|
Target: 5'- --cCAGG-GCCAcCAGCAGCuGGaAGCCc -3' miRNA: 3'- gucGUCCuCGGU-GUUGUCGuCC-UCGG- -5' |
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| 13345 | 3' | -57 | NC_003409.1 | + | 21769 | 0.68 | 0.686472 |
|
Target: 5'- aGGCAGucAGCacaGCGACgAGCAGGAuGCCc -3' miRNA: 3'- gUCGUCc-UCGg--UGUUG-UCGUCCU-CGG- -5' |
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| 13345 | 3' | -57 | NC_003409.1 | + | 24271 | 0.79 | 0.18758 |
|
Target: 5'- gCAGCaccccAGGAGCCcCGGCAGCaccccAGGAGCCc -3' miRNA: 3'- -GUCG-----UCCUCGGuGUUGUCG-----UCCUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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