Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13347 | 5' | -47.8 | NC_003409.1 | + | 13993 | 0.73 | 0.923288 |
Target: 5'- cGAGCCACCGGGaACUg-----UGCg- -3' miRNA: 3'- aCUCGGUGGUCC-UGAauuuaaACGac -5' |
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13347 | 5' | -47.8 | NC_003409.1 | + | 24736 | 0.74 | 0.882671 |
Target: 5'- gGGGCgCGCCGGGGCUccuGGggUGCUGc -3' miRNA: 3'- aCUCG-GUGGUCCUGAau-UUaaACGAC- -5' |
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13347 | 5' | -47.8 | NC_003409.1 | + | 127570 | 1.11 | 0.010568 |
Target: 5'- gUGAGCCACCAGGACUUAAAUUUGCUGg -3' miRNA: 3'- -ACUCGGUGGUCCUGAAUUUAAACGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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